發(fā)布時(shí)間：2018-07-14 21:43

【摘要】：為探明茶樹抗寒轉(zhuǎn)錄因子基因CsCBF1與CsICE1的表達(dá)特性,以茶樹幼苗為試驗(yàn)材料,根據(jù)其他已知植物的抗寒轉(zhuǎn)錄因子基因CBF1與ICE1序列,通過(guò)同源克隆法獲得茶樹中相對(duì)應(yīng)的同源基因CsCBF1與CsICE1的保守區(qū)域,長(zhǎng)度分別為456 bp與643 bp,經(jīng)比對(duì)確定為CBF與ICE轉(zhuǎn)錄因子家族基因中CsCBF1與CsICE1型基因,因此命名為CsCBF1與CsICE1。熒光定量PCR分析CsCBF1與CsICE1基因在冷脅迫條件下的表達(dá)情況,發(fā)現(xiàn)其在4℃處理8 h時(shí)表達(dá)量最高;在茶樹葉片中表達(dá)量顯著高于根、莖、花。低溫下CsCBF1與CsICE1基因表達(dá)顯著增加,并且在抗寒性較強(qiáng)的種質(zhì)(0306I和黃旦)中的表達(dá)顯著高于抗寒性相對(duì)較弱的種質(zhì)(0306F和0306D)。本研究結(jié)果為進(jìn)一步探究CsCBF1與CsICE1基因在茶樹耐低溫脅迫過(guò)程中基因表達(dá)的調(diào)控機(jī)制奠定了基礎(chǔ)。
[Abstract]:In order to investigate the expression characteristics of CsCBF1 and CsICE1 genes in tea plants, the chilling resistant transcription factor genes CBF1 and ICE1 sequences of other known plants were used as experimental materials. The conserved regions of the homologous genes CsCBF1 and CsICE1 in tea plants were obtained by homologous cloning. The length of CsCBF1 and CsICE1 were 456 BP and 643 BP, respectively, which were identified as CsCBF1 and CsICE1 type genes in CBF and ICE transcription factor family genes, so they were named CsCBF1 and CsICE1. The expression of CsCBF1 and CsICE1 genes under cold stress was analyzed by fluorescence quantitative PCR. It was found that CsCBF1 and CsICE1 gene expression was the highest at 4 鈩，

本文編號(hào)：2123044