本文選題：凡納濱對(duì)蝦 + 幼蝦�。� 參考：《上海海洋大學(xué)》2017年碩士論文

【摘要】：為探究凡納濱對(duì)蝦養(yǎng)殖2-3月齡階段的生長(zhǎng)差異、生長(zhǎng)相關(guān)基因及生長(zhǎng)標(biāo)記,本實(shí)驗(yàn)通過(guò)采集對(duì)蝦生長(zhǎng)第28、38、48、58、68、73天的凡納濱對(duì)蝦,共取樣857只。按照生長(zhǎng)速率不同將體重排名的前10%(14只)、中間10%(14只)、最后10%(14只)分為生長(zhǎng)速率高中低三組。根據(jù)統(tǒng)計(jì)量用冪函數(shù)W=aLb進(jìn)行體長(zhǎng)體重生長(zhǎng)關(guān)系的擬合;體長(zhǎng)體重取對(duì)數(shù),確定所有樣品在內(nèi)及不同生長(zhǎng)速率組最佳直線方程;同時(shí)計(jì)算不同生長(zhǎng)速率組對(duì)蝦的絕對(duì)日增長(zhǎng)、絕對(duì)日增重和相對(duì)日增長(zhǎng)率、相對(duì)日增重率,以探究不同生長(zhǎng)速率組對(duì)蝦的生長(zhǎng)差異。通過(guò)2-ΔΔCT方法對(duì)不同生長(zhǎng)速率組不同生長(zhǎng)時(shí)期的樣品進(jìn)行RPL18、SNRPG、RPL7基因相對(duì)表達(dá)量的分析。最后以28天的樣品對(duì)多態(tài)性較好的15對(duì)微衛(wèi)星引物進(jìn)行體重與位點(diǎn)相關(guān)性的分析。結(jié)果發(fā)現(xiàn):首先,相同生長(zhǎng)時(shí)期的三組蝦生長(zhǎng)速率顯著差異(P95%),將這些生長(zhǎng)速率不同的蝦作為一個(gè)整體來(lái)分析蝦的生長(zhǎng)情況可能會(huì)出現(xiàn)一定偏差。其次,體長(zhǎng)與體重的回歸結(jié)果為:冪函數(shù)W所有樣品=1χ10-5L3.0051,直線方程y所有樣品=3x-4.92,不同生長(zhǎng)速率組的直線方程分別為y高=3.41x-5.71,y中=3.21x-5.30,y低=2.92x-4.78;直線方程中斜率與生長(zhǎng)速率呈正相關(guān),三組線性回歸方程能更加確切地反映出三組蝦的真實(shí)生長(zhǎng)情況;同時(shí)發(fā)現(xiàn),不同生長(zhǎng)速率蝦的絕對(duì)生長(zhǎng)(絕對(duì)日增長(zhǎng)和絕對(duì)日增重)符合對(duì)蝦的實(shí)際生長(zhǎng)情況,而相對(duì)生長(zhǎng)(相對(duì)日增長(zhǎng)率和相對(duì)日增重率)與對(duì)蝦的實(shí)際生長(zhǎng)情況相反,建議以后分析對(duì)蝦生長(zhǎng)特性時(shí)參數(shù)選擇絕對(duì)生長(zhǎng)值更為適宜。對(duì)于表達(dá)量的研究表明,不同生長(zhǎng)速率組RPL18、SNRPG基因的相對(duì)表達(dá)量差異不顯著(P0.05),但除28天外,均表現(xiàn)為高速率組低速率組;RPL7基因相對(duì)表達(dá)量與體長(zhǎng)的相關(guān)性高于體重,并且均表現(xiàn)為低速率組高中速率組,推測(cè)RPL7基因極大可能的參與凡納濱對(duì)蝦生長(zhǎng)的負(fù)調(diào)控。本研究得出與體重存在相關(guān)性的TUMXLV 10.284微衛(wèi)星引物,結(jié)果表明BB基因型為高速率組和中速率組中的優(yōu)勢(shì)基因型(平均體重:3.39±0.37g、2.54±0.30 g),AC、CC為低速率組中的優(yōu)勢(shì)基因型(平均體重:0.53±0.10g、0.98±0.3g);所有樣品在內(nèi)的數(shù)據(jù)顯示BB、AB基因型對(duì)應(yīng)的體重(2.85±0.31 g、2.73±0.51g)最高,AC、CC基因型對(duì)應(yīng)的體重(1.18±1.12 g、1.45±0.36g)最低,且體重差異顯著(P0.05)。并且發(fā)現(xiàn)帶B基因型的個(gè)體體重均較高,帶C基因型的個(gè)體體重均較低,且差異顯著(P0.05)。
[Abstract]:In order to investigate the growth difference, growth related genes and growth markers of Penaeus vannamei cultured in 2-3 months of age, 857 prawns were collected from the 28 ~ (38) ~ (th) ~ (48) ~ (th) ~ (-) day of the growth of Penaeus vannamei (P.vannamei) in this experiment. According to the growth rate, the top 10% (14), the middle 10% (14) and the last 10% (14) were divided into three groups. According to the statistics, the relationship between body length and body weight was fitted by power function Wlb; the logarithm of body weight was taken to determine the best linear equation for all samples and groups of different growth rates; and the absolute daily growth of Penaeus penaeus at different growth rates was calculated. Absolute daily gain, relative daily growth rate and relative daily gain rate were used to explore the growth differences of shrimp in different growth rates. The relative expression of RPL18, SNRPGN RPL7 gene in different growth rate groups was analyzed by 2- 螖 CT. At last, 15 pairs of microsatellite primers with good polymorphism were used to analyze the correlation between body weight and locus. The results showed that: first, the growth rate of the three groups of shrimp in the same growth period was significantly different (P95%). Secondly, the regression results of body length and body weight were as follows: power function W all samples 蠂 ~ (-1) X ~ (-5) L ~ (3.0051), linear equation y _ y all samples ~ (3x-4.92), the linear equations of different growth rate groups were y ~ (3.41) -5.71 ~ (-1) y low 2.922x-4.78, and the slope of the linear equation was positively correlated with growth rate. Three groups of linear regression equations can more accurately reflect the actual growth of the three groups of shrimp, and found that the absolute growth (absolute daily growth and absolute daily gain) of shrimp at different growth rates is in line with the actual growth of Penaeus Monodon. However, the relative growth (relative daily growth rate and relative daily gain rate) is contrary to the actual growth of Penaeus Monodon. It is suggested that it is more appropriate to choose the absolute growth value when analyzing the growth characteristics of Penaeus penaeus in the future. The results showed that there was no significant difference in the relative expression of RPL18SN-SNRPG gene among different growth rate groups (P0.05), but the correlation between the relative expression of RPL7 gene and body length was higher than that of body length in high rate group and low rate group, except for 28 days. The RPL7 gene may be involved in the negative regulation of the growth of Penaeus vannamei. In this study, the microsatellite primers TUMXLV 10.284 correlated with body weight were obtained. The results showed that BB genotype was the dominant genotype in high rate group and middle rate group (mean weight: 3.39 鹵0.37 g / 2.54 鹵0.30 g). ACCC was the dominant genotype in low rate group (mean weight was 0.53 鹵0.10 g / 0.98 鹵0.3 g), and all samples showed that the weight of BBAB genotype corresponded to that of BBAB genotype. (2.85 鹵0.31 g / L 2.73 鹵0.51g) the highest weight corresponding to the ACCC genotype was the lowest (1.18 鹵1.12 g / L 1.45 鹵0.36 g). And the weight difference was significant (P0.05). It was found that the individuals with genotype B and genotype C had higher body weight and lower body weight than those with genotype C, and the difference was significant (P0.05).
【學(xué)位授予單位】：上海海洋大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S968.22

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