本文選題：桃樹(shù) + PpSnRKβλ��； 參考：《北方園藝》2017年11期

【摘要】：以實(shí)生毛桃為試材,采用PCR和實(shí)時(shí)熒光定量技術(shù),克隆桃樹(shù)SnRK1蛋白激酶(蔗糖非發(fā)酵-1-型相關(guān)蛋白激酶-1)的調(diào)節(jié)亞基βλ,并分析其組織表達(dá)特性。構(gòu)建p35S::PpSnRK1βγ1重組載體,獲得超表達(dá)PpSnRK1βλ1基因擬南芥植株用于分析其生物功能。結(jié)果表明:克隆獲得桃樹(shù)SnRK1βλ1,命名為PpSnRK1βλ1。該cDNA全長(zhǎng)為1 476bp,編碼492個(gè)氨基酸。序列分析表明,其包含CBM和4個(gè)CBS結(jié)構(gòu)域;PpSnRK1βλ1與果梅的SnRK1βλ蛋白親緣關(guān)系最近;PpSnRK1βλ1基因在實(shí)生毛桃的根、莖、葉均有表達(dá),其中在莖中表達(dá)量最低。超表達(dá)PpSnRK1βγ1基因擬南芥株系A(chǔ)-βγ1的花期比野生型晚2.19d,單株蓮座葉數(shù)量比野生型多1.17片;葉片的葉綠素、可溶性糖和可溶性蛋白質(zhì)含量均顯著高于野生型擬南芥,分別比野生型提高了13.7%、12.9%和23.3%,但淀粉含量卻沒(méi)有顯著性差異。在氧化脅迫條件下,轉(zhuǎn)基因植株與野生型的生長(zhǎng)均受到抑制,但前者具有更好的萌發(fā)率和主根長(zhǎng)度,以保證植株正常生長(zhǎng)。因此,PpSnRK1βλ1基因參與調(diào)控植株的碳氮代謝,并影響植物的花期,以及在防御氧化脅迫過(guò)程中起重要作用。
[Abstract]:The regulation subunit 尾 位 of SnRK1 protein kinase (sucrose non-fermentation-associated protein kinase 1) was cloned by PCR and real-time fluorescence quantitative technique, and its tissue expression characteristics were analyzed. P35 S: PpSnRK1 尾 緯 1 recombinant vector was constructed, and the overexpression of PpSnRK1 尾 位 1 gene was obtained from Arabidopsis thaliana to analyze its biological function. The results showed that SnRK1 尾 位 1 was cloned and named PpSnRK1 尾 位 1. The cDNA is 1476 BP and encodes 492 amino acids. Sequence analysis showed that PpSnRK1 尾 位 1 containing CBM and four CBS domains was closely related to the SnRK1 尾 位 1 protein of fruit plum. PpSnRK1 尾 位 1 gene was expressed in roots, stems and leaves of Phyllostachys pubescens, and the lowest expression was found in stems. In Arabidopsis thaliana strain A- 尾 緯 1 with overexpression of PpSnRK1 尾 緯 1 gene, the flowering time of A- 尾 緯 1 strain was 2.19 days later than that of wild type, the number of rosette leaves per plant was 1.17 more than that of wild type, and the contents of chlorophyll, soluble sugar and soluble protein in leaves of Arabidopsis thaliana were significantly higher than those of wild type Arabidopsis thaliana. Compared with wild type, the content of starch increased by 13.9% and 23.3%, respectively, but there was no significant difference in starch content. Under the condition of oxidative stress, the growth of transgenic plants and wild-type plants were inhibited, but the former had better germination rate and main root length to ensure the normal growth of the plants. Therefore, PpSnRK1 尾 位 1 gene plays an important role in regulating the metabolism of carbon and nitrogen in plants, affecting the flowering period of plants, and in the process of defense against oxidative stress.
【作者單位】： 山東農(nóng)業(yè)大學(xué)園藝科學(xué)與工程學(xué)院作物生物學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專(zhuān)項(xiàng)資金資助項(xiàng)目(CARS-31)
【分類(lèi)號(hào)】：S662.1
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本文編號(hào)：2114559