本文選題：基因克隆 + 生物信息學(xué)分析��； 參考：《植物生理學(xué)報》2017年09期

【摘要】：磷脂:二酰甘油酰基轉(zhuǎn)移酶(PDAT)能夠催化磷脂酰膽堿(PC)sn-2位的�；D(zhuǎn)移至二酰甘油(DAG)上,形成三酰甘油(TAG)和溶血磷脂酰膽堿,這是另外一條不同于Kennedy途徑的三酰甘油合成方式,故明確油茶(Camellia oleifera)PDAT基因的功能對闡明油茶種子油脂合成機制具有重要意義。本研究通過cDNA末端快速擴增(RACE)技術(shù)克隆油茶CoPDAT基因的全長cDNA序列,該序列長2773 bp,開放閱讀框為2022 bp,編碼673個氨基酸。亞細胞定位分析確定CoPDAT基因所編碼蛋白質(zhì)定位于內(nèi)質(zhì)網(wǎng)上。實時熒光定量PCR分析結(jié)果表明,CoPDAT在油茶不同組織/器官中均有表達,在種子中不同發(fā)育時期的相對表達量呈"上升-下降-上升-下降-上升"的表達特征,在花后42周表達量最高。CoPDAT基因與油茶種子油脂含量的灰色關(guān)聯(lián)度值為0.8356,表明該基因與油茶種子油脂的合成積累關(guān)系密切。本研究為闡明CoPDAT基因在油茶油脂合成過程中的功能以及為油茶的分子遺傳改良提供參考。
[Abstract]:Phospholipid: diacylglycerol transferase (PDAT) catalyzes the transfer of acyl groups from the sn-2 site of phosphatidylcholine (PC) to diacylglycerol (DAG) to form triglycerides (tag) and lysophosphatidylcholine (lysophosphatidylcholine). Therefore, it is important to clarify the function of Camellia oleifera PDAT gene in oil synthesis mechanism of Camellia oleifera. In this study, the full-length cDNA sequence of Camellia oleifera CoPDAT gene was cloned by rapid amplification of cDNA terminal (race). The length of the cDNA was 2773 BP, and the open reading frame was 2022 BP, encoding 673 amino acids. Subcellular localization analysis confirmed that the protein encoded by CoPDAT gene was located on the endoplasmic reticulum. The results of real-time fluorescence quantitative PCR analysis showed that CoPDAT was expressed in different tissues / organs of Camellia oleifera, and the relative expression level of CoPDAT in different development stages of Camellia oleifera was "upper-down-ascendant". At 42 weeks after anthesis, the highest expression of .CoPDAT gene and the grey-related degree of oil content of Camellia oleifera seeds were 0.8356, which indicated that this gene was closely related to the synthesis and accumulation of oil in Camellia oleifera seeds. This study was intended to elucidate the function of CoPDAT gene in the synthesis of Camellia oleifera oil and provide a reference for the molecular genetic improvement of Camellia oleifera.
【作者單位】： 中南林業(yè)科技大學(xué)經(jīng)濟林培育與保護教育部重點實驗室經(jīng)濟林育種與栽培國家林業(yè)局重點實驗室經(jīng)濟林培育與利用湖南省2011協(xié)同創(chuàng)新中心;
【基金】：國家自然科學(xué)基金(31470684)~~
【分類號】：Q943.2;S794.4
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本文編號：2110183