本文選題：池蝶蚌 + Hsβ-catenin　； 參考：《水生生物學(xué)報》2017年05期

【摘要】：為了解淡水貝類性別調(diào)控與分化機(jī)制,課題組建立了池蝶蚌(Hyriopsis schlegelii)性腺轉(zhuǎn)錄組,在轉(zhuǎn)錄組庫中,存在β-連環(huán)蛋白(β-catenin)基因序列。實驗對池蝶蚌β-catenin基因進(jìn)行驗證,采用RACE技術(shù)克隆其c DNA全長,命名為Hsβ-catenin。該序列全長4386 bp,5′-非編碼區(qū)為162 bp,3′-非編碼區(qū)為1758 bp,開放閱讀框為2466 bp,編碼821個氨基酸;該蛋白結(jié)構(gòu)域主要由12個ARM重復(fù)序列組成;二級結(jié)構(gòu)中,α-螺旋占47.75%,β-折疊占1.22%,隨機(jī)卷曲占51.04%;三級結(jié)構(gòu)中含大量α-螺旋且為右手超螺旋,構(gòu)成ARM結(jié)構(gòu)域;系統(tǒng)進(jìn)化樹分析表明,Hsβ-catenin與軟體動物聚為一支,然后與昆蟲類聚為一支。實時熒光定量PCR(q RT-PCR)檢測顯示,Hsβ-catenin在腸中表達(dá)量最高,其次是斧足和精巢。Hsβ-catenin基因在12月齡和36月齡表達(dá)量較高,且在36月齡表達(dá)量最高,表明其可能參與池蝶蚌的性別調(diào)控與分化作用。
[Abstract]:In order to understand the sex regulation and differentiation mechanism of freshwater shellfish, the gonadal transcriptome of Hyriopsis schlegelii was established. There was a 尾 -catenin gene sequence in the transcriptional library. The cDNA of 尾 -catenin gene was cloned by race technique and named as Hs 尾 -catenin. The total length of the sequence is 4386 BP / 5, and the non-coding region is 162bp / 3G / L, which is 1758 BP and the open reading frame is 2466 BP, which encodes 821 amino acids, and the protein domain is mainly composed of 12 arm repeats. Among the secondary structures, 偽 -helix accounts for 47.75, 尾 -fold for 1.22, random crimp for 51.044.The tertiary structure contains a large number of 偽 -helix and right hand superhelix, which forms the arm domain. Phylogenetic tree analysis shows that Hs 尾 -catenin is one with mollusks and then with insects. The expression of Hs 尾 -catenin was the highest in the intestine by real-time fluorescence quantitative PCR (Q RT-PCR), followed by the expression of Hs 尾 -catenin gene at 12 and 36 months of age and the highest expression at 36 months old. The results suggested that it might be involved in the sex regulation and differentiation.
【作者單位】： 南昌大學(xué)生命科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金(31660337) 江西省教育廳項目(GJJ150166)資助~~
【分類號】：S917.4
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本文編號：2109299