本文選題：麒麟雞 + 卷羽�。� 參考：《基因組學(xué)與應(yīng)用生物學(xué)》2017年03期

【摘要】：麒麟雞(frizzle chicken)是我國(guó)亞熱帶地區(qū)特有的一種具有卷羽特性的優(yōu)質(zhì)肉雞品種。本課題組前期研究發(fā)現(xiàn)在麒麟雞皮膚中高度表達(dá)miRNA-1623(miR-1623),因此推測(cè)其參與麒麟雞毛囊發(fā)育及卷羽的形成過程。為研究其獨(dú)特的毛囊發(fā)育及卷羽散熱機(jī)制,本研究利用生物信息學(xué)手段根據(jù)其靶標(biāo)結(jié)合位點(diǎn)尋找到其潛在靶標(biāo)Wnt4基因,并利用qRT-PCR對(duì)卷羽麒麟雞和片羽懷鄉(xiāng)雞中的miR-1623與Wnt4的表達(dá)水平進(jìn)行了檢測(cè)。進(jìn)一步通過構(gòu)建雙熒光素酶報(bào)告基因pmirGlo載體在細(xì)胞水平對(duì)miR-1623與Wnt4的靶標(biāo)關(guān)系進(jìn)行了驗(yàn)證。結(jié)果表明:Wnt4 mRNA的3'UTR存在miR-1623靶標(biāo)結(jié)合位點(diǎn)(TGCCTG);相對(duì)于懷鄉(xiāng)雞,麒麟雞皮膚中miR-1623表達(dá)水平顯著升高(p0.01),Wnt4 mRNA表達(dá)水平顯著降低(p0.01);雙熒光素酶檢測(cè)分析發(fā)現(xiàn)miR-1623靶向負(fù)調(diào)控Wnt4-3'UTR。本實(shí)驗(yàn)可為進(jìn)一步揭示miRNA-1623在禽類毛囊生長(zhǎng)發(fā)育過程中的作用提供參考。
[Abstract]:Steamed Sliced Chicken with Mushrooms and Vegetables (frizzle chicken) is a special high quality broiler breed in subtropical region of China. Our previous study found that miRNA-1623 (miR-1623) is highly expressed in the skin of Steamed Sliced Chicken with Mushrooms and Vegetables. Therefore, we speculate that miRNA-1623 is involved in the development of Steamed Sliced Chicken with Mushrooms and Vegetables hair follicles and the formation of curly feathers. In order to study its unique mechanism of hair follicle development and heat dissipation, we used bioinformatics to find its potential target Wnt4 gene according to its target binding site. The expression levels of miR-1623 and Wnt4 were detected by qRT-PCR in curly feather Steamed Sliced Chicken with Mushrooms and Vegetables and Shiyu Huai Xiang chicken. Furthermore, the targeting relationship between miR-1623 and Wnt4 was verified at the cell level by constructing a double luciferase reporter gene pmirGlo vector. The results showed that there was a miR-1623 target binding site (TGCCTG) in 3UTR of Wnt4 mRNA, a significant increase in miR-1623 mRNA expression level in Steamed Sliced Chicken with Mushrooms and Vegetables skin (p0.01) and a significant decrease in Wnt4 mRNA expression level (p0.01) in Steamed Sliced Chicken with Mushrooms and Vegetables skin, and a negative regulation of Wnt4-3UTR targeting miR-1623 was found by double luciferase assay. This study may provide a reference for further revealing the role of miRNA-1623 in the growth and development of poultry hair follicles.
【作者單位】： 廣東海洋大學(xué)動(dòng)物科學(xué)系;中國(guó)農(nóng)業(yè)科學(xué)院特產(chǎn)研究所特種經(jīng)濟(jì)動(dòng)物分子生物學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室;
【基金】：廣東省創(chuàng)新強(qiáng)校重大項(xiàng)目(GDOU2013050222) 國(guó)家科技基礎(chǔ)條件平臺(tái)-特種經(jīng)濟(jì)種質(zhì)資源共享子平臺(tái)(2016020)共同資助
【分類號(hào)】：S831

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