本文選題：乙型腦炎病毒 + 基因Ⅰ型��； 參考：《畜牧獸醫(yī)學報》2017年05期

【摘要】：為了評估豬基因Ⅰ型乙型腦炎病毒YA1株、CZ株滅活疫苗的免疫原性,通過將YA1株、CZ株乙腦病毒分別在乳鼠腦內連續(xù)傳代,獲得了高病毒含量的鼠腦病毒液。將佐劑ISA206分別與滅活的YA1株、CZ株乙腦病毒液混合,制備成鼠腦滅活苗。將YA1株、CZ株鼠腦滅活苗以及HW1株商品化鼠腦滅活苗分別免疫接種小鼠、豚鼠、仔豬,采用間接ELISA試驗、血凝抑制試驗、病毒中和試驗以及攻毒保護試驗分別檢測IgG抗體、血凝抑制抗體、中和抗體的的產(chǎn)生情況以及疫苗對小鼠的攻毒保護率。結果顯示YA1株、CZ株滅活苗在小鼠和仔豬上產(chǎn)生的IgG抗體、在豚鼠上產(chǎn)生的血凝抑制抗體、在仔豬上產(chǎn)生的中和抗體以及對小鼠的攻毒保護率均高于HW1株。結果表明乙腦病毒YA1株、CZ株滅活苗的免疫原性優(yōu)于HW1株,其中CZ株滅活苗的免疫原性最優(yōu)。
[Abstract]:In order to evaluate the immunogenicity of the inactivated vaccine against porcine type I encephalitis virus strain YA1 strain CZ strain of Japanese encephalitis virus was subcultured in the brain of the young mice to obtain the high content of the virus in the brain. The inactivated vaccine was prepared by mixing the adjuvant ISA206 with the inactivated YA1 strain CZ strain Japanese encephalitis virus. Mice, guinea pigs and piglets were inoculated with inactivated brain vaccine of YA1 strain CZ strain and commercial inactivated vaccine HW1 strain respectively. IgG antibody was detected by indirect Elisa test, hemagglutination inhibition test, virus neutralization test and attack virus protection test. Hemagglutination inhibition antibody, neutralizing antibody production and the protective rate of the vaccine to mice. The results showed that the IgG antibody produced by inactivated YA1 strain CZ strain on mice and piglets, hemagglutination inhibitory antibody on guinea pig, neutralizing antibody on piglets and the protective rate against HW1 strain were higher than those of HW1 strain. The results showed that the immunogenicity of inactivated vaccine of Japanese encephalitis virus YA1 strain CZ strain was superior to that of HW1 strain, and the immunogenicity of inactivated vaccine of CZ strain was the best.
【作者單位】： 四川農業(yè)大學動物醫(yī)學院豬病研究中心;
【基金】：公益性行業(yè)(農業(yè))科研專項(201203082)
【分類號】：S858.28

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