本文選題：脾虛號方 + 功能性消化不良　； 參考：《北京中醫(yī)藥大學(xué)學(xué)報》2017年09期

【摘要】：目的探討功能性消化不良(FD)脾虛證大鼠胃體組織肌球蛋白輕鏈(MLC)蛋白與mRNA表達(dá)及脾虛1號方的干預(yù)作用。方法 60只7 d齡雄性SD大鼠隨機(jī)分為正常對照組(n=10)、FD脾虛證模型組(n=50)。正常組給予2%蔗糖溶液灌胃,FD脾虛證模型組給予0.1%蔗糖碘乙酰胺溶液灌胃,0.2 m L/(只·d),連續(xù)6 d。FD脾虛證模型組正常飼料喂養(yǎng)至6周齡后疊加改良小平臺站立,連續(xù)14 d。造模結(jié)束后隨機(jī)分為模型組、多潘立酮組、脾虛1號方低、中、高劑量組,各10只,連同正常組,分別給予蒸餾水10 m L/(kg·d)、多潘立酮3.125 mg/(kg·d)、脾虛1號方1.275、2.550、5.100 g/(kg·d),灌胃14 d。采用免疫組化、蛋白質(zhì)印跡(Western blot)和RT-PCR方法檢測胃體組織MLC蛋白及mRNA表達(dá)量。結(jié)果與正常組相比,免疫組化檢測模型組大鼠胃體肌層MLC蛋白定位表達(dá)降低;Western blot檢測模型組胃體組織MLC蛋白表達(dá)量降低;RT-PCR檢測模型組大鼠胃體組織MLC mRNA表達(dá)量降低,差異均有統(tǒng)計學(xué)意義(P0.01)。與模型組相比,免疫組化檢測脾虛1號方低、中、高劑量組大鼠胃體肌層MLC蛋白定位表達(dá)增加,差異均有統(tǒng)計學(xué)意義(P0.05,P0.01);Western blot檢測脾虛1號方中、高劑量組大鼠胃體MLC蛋白表達(dá)量增加,差異均有統(tǒng)計學(xué)意義(P0.01);RT-PCR檢測脾虛1號方高劑量組MLC mRNA表達(dá)量升高,差異有統(tǒng)計學(xué)意義(P0.01)。結(jié)論 FD脾虛證模型大鼠存在胃體組織MLC蛋白及基因表達(dá)的降低,而脾虛1號方能夠上調(diào)MLC蛋白及基因的表達(dá)。
[Abstract]:Objective to investigate the expression of myosin light chain (MLC) protein and mRNA in gastric body of rats with functional dyspepsia (FD) spleen deficiency syndrome and the intervention effect of spleen deficiency prescription No. 1. Methods 60 male SD rats aged 7 days were randomly divided into normal control group (n = 10) and model group of FD spleen deficiency syndrome (n = 50). The normal group was given 2% sucrose solution by gastric perfusion with FD spleen deficiency syndrome model group. The model group was given 0.1% sucrose iodide acetamide solution intragastric perfusion with 0.2 mL / (d), continuous 6 d.FD spleen deficiency model group). The normal diet was fed to 6 weeks old and the modified small platform was added to stand up for 14 days. After the model was made, the rats were randomly divided into two groups: model group, doperidone group, low, medium and high dose groups, 10 rats in each group, and 10 rats in each group were given distilled water 10 mL / (kg d), doperidone 3.125 mg/ (kg d), spleen deficiency 1 prescription 1.275U 2.550 ~ 5.100g / (kg d), for 14 days. Immunohistochemistry, Western blot and RT-PCR were used to detect the expression of MLC protein and mRNA in gastric body. Results compared with the normal group, the localization expression of MLC protein in the gastric body myometrium of the model group was detected by immunohistochemistry. The expression of MLC protein in the gastric body tissue of the model group was detected by Western blot. The expression of MLC mRNA in the gastric body tissue of the model group was detected by RT-PCR, and the expression of MLC mRNA in the gastric body of the model group was detected by RT-PCR. The difference was statistically significant (P0.01). Compared with the model group, the expression of MLC protein in the gastric muscle layer of the rats in the high dose group was significantly higher than that in the model group. The expression of MLC protein in the gastric body myometrium of the rats in the high dose group was higher than that in the model group, and the difference was statistically significant (P0.05 P0.01). The expression of MLC protein increased in high dose group (P0.01). The expression of MLC mRNA in high dose group (P0.01) was significantly higher than that in high dose group (P0.01). Conclusion there is a decrease in MLC protein and gene expression in gastric body in FD spleen deficiency model rats, while spleen deficiency Formula 1 can upregulate MLC protein and gene expression.
【作者單位】： 中國中醫(yī)科學(xué)院西苑醫(yī)院消化科北京市中醫(yī)脾胃病研究所;中國中醫(yī)科學(xué)院研究生院;
【基金】：國家重點(diǎn)基礎(chǔ)研究發(fā)展計劃(973計劃)資助項(xiàng)目(No.2013CB531703) 國家自然科學(xué)基金資助項(xiàng)目(No.81503567) 中國博士后科學(xué)基金資助項(xiàng)目(No.2015M1227,2016T90195)~~
【分類號】：R285.5

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