本文選題：電針 + 高血壓前期��； 參考：《北京中醫(yī)藥大學(xué)》2017年碩士論文

【摘要】：[研究目的]現(xiàn)代生活節(jié)奏的加快,工作壓力和社會(huì)競(jìng)爭(zhēng)性的增強(qiáng),越來(lái)越多的人長(zhǎng)期處于精神緊張焦慮狀態(tài)使得應(yīng)激性高血壓的患病率逐年增加。2003年美國(guó)JNC-7第一次提出"高血壓前期(Prehypertension)"即收縮壓在120-139mmHg之間或舒張壓在80-89 mmHg之間的階段。研究表明高血壓前期與心血管事件的發(fā)生呈正相關(guān),甚至?xí)_(kāi)始有血管,心,腦,腎等靶器官損害。針刺干預(yù)可以在高血壓前期這個(gè)關(guān)鍵階段有效遏制高血壓的發(fā)生并對(duì)腎臟等靶器官進(jìn)行保護(hù),為維護(hù)人類健康具有重要意義。本研究利用基因芯片技術(shù)、生物信息學(xué)分析技術(shù)深入探究電針對(duì)應(yīng)激性高血壓前期的干預(yù)效果及其干預(yù)機(jī)制,為高血壓病的防治提供新的依據(jù)。[研究方法]將36只雄性Wistar大鼠(9周齡,220±30g)隨機(jī)分為空白組,模型組和電針+模型3組,每組12只。運(yùn)用足底電擊結(jié)合噪聲的刺激方法對(duì)模型組與電針組大鼠進(jìn)行11天的造模,制備應(yīng)激性高血壓前期大鼠模型,并對(duì)電針組實(shí)施電針干預(yù)。分別在電針治療前以及接受刺激后的第3、5、7、9、11天測(cè)量三組大鼠收縮壓,并于造模和治療結(jié)束后進(jìn)行取材,采用Gene Chip Rat Gene 2.0 ST芯片技術(shù)檢測(cè)三組大鼠腎組織基因表達(dá)譜的改變,篩選出差異表達(dá)基因(P0.05,Fold Changes1.5 or Fold Changes =-1.5)。運(yùn)用 GO 功能富集分析(Gene Ontology)和 KEGG 通路富集分析(Kyoto Encyclopedia of Genes and Genomes)等生物信息學(xué)分析技術(shù)對(duì)差異基因進(jìn)行生物學(xué)功能與通路的富集分析。[實(shí)驗(yàn)結(jié)果]造模開(kāi)始后,空白組血壓保持穩(wěn)定;第3天,模型組血壓開(kāi)始上升120mm Hg,至第11天,血壓持續(xù)升高(P0.01)。針刺干預(yù)第5天開(kāi)始,針刺組血壓低于模型組(P0.05)。針刺組血壓雖然沒(méi)有降至正常,但與模型組相比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。基因組注釋(Genome annotation)是利用生物信息學(xué)方法和工具,對(duì)基因組所有基因的生物學(xué)功能進(jìn)行高通量注釋。GO功能富集分析把所有的差異表達(dá)基因映射到GO數(shù)據(jù)庫(kù)的每個(gè)條目上,通過(guò)計(jì)算確定每個(gè)條目所對(duì)應(yīng)的差異表達(dá)基因的數(shù)目,給出兩樣本間差異表達(dá)基因所參與的主要生物學(xué)功能,發(fā)現(xiàn)電針組與模型組的差異基因與氯離子通道復(fù)合體復(fù)雜離子通道,氯離子結(jié)合,氯離子通道活性,受體活性等功能有關(guān)。KEGG信號(hào)通路分析顯示模型組有7條通路被激活,與模型組比較電針組有2條通路被激活。經(jīng)文獻(xiàn)檢索發(fā)現(xiàn)神經(jīng)受體通路(Neuroactive ligand-receptor action)與高血壓發(fā)病及電針降壓密切相關(guān),并有GABRB3基因在此條通路上表達(dá)。根據(jù)電針組差異表達(dá)基因的分析可見(jiàn)GABRB3,CFTR,CISH基因,常出現(xiàn)在富集功能和信號(hào)通路中,經(jīng)文獻(xiàn)檢索發(fā)現(xiàn)這3個(gè)基因和高血壓的發(fā)病密切相關(guān)。電針降壓機(jī)制可能是通過(guò)參與調(diào)控這3個(gè)關(guān)鍵基因的功能表達(dá)及其信號(hào)轉(zhuǎn)導(dǎo)通路來(lái)實(shí)現(xiàn).[實(shí)驗(yàn)結(jié)論]1.對(duì)應(yīng)激性的高血壓前期大鼠進(jìn)行電針曲池和太沖穴的干預(yù)有降壓效應(yīng)。2.電針曲池和太沖穴影響應(yīng)激性的高血壓前期大鼠腎組織基因表達(dá)譜。3.電針曲池和太沖穴可能通過(guò)GABRB3,CFTR,CISH基因的調(diào)控和神經(jīng)受體活性通路(Neuroactive ligand-receptor action)降壓。
[Abstract]:[Objective] to accelerate the pace of modern life, work stress and social competitiveness, more and more people are in a state of prolonged mental anxiety and anxiety. The prevalence of stress hypertension has increased year by year in.2003, American JNC-7 first proposed "prehypertension (Prehypertension)" that is, systolic pressure is between 120-139mmHg or diastolic pressure. 80-89 mmHg stage. The study shows that prehypertension is positively related to the occurrence of cardiovascular events, and may even begin to damage target organs such as blood vessels, heart, brain and kidney. Acupuncture intervention can effectively restrain the occurrence of hypertension and protect the kidney and other target organs at this critical stage of prehypertension, which is important for the maintenance of human health. This study used gene chip technology and bioinformatics analysis technique to explore the intervention effect and intervention mechanism of electroacupuncture corresponding to pre induced hypertension in order to provide a new basis for the prevention and treatment of hypertension. [research methods] randomly divided 36 male Wistar rats (9 weeks old, 220 + 30g) into blank group, model group and electroacupuncture + model 3. In group 12, the model group and the electroacupuncture group were made for 11 days by the stimulation of the foot shock and the noise. The stress prehypertension rat model was prepared and the electroacupuncture intervention was carried out in the electroacupuncture group. The systolic pressure of three groups of rats was measured before the electroacupuncture treatment and the 3,5,7,9,11 day after the stimulation, and the model and treatment were made and treated. After the treatment, Gene Chip Rat Gene 2 ST chip was used to detect the changes of gene expression profiles in the renal tissue of three groups of rats, and the differential expression genes (P0.05, Fold Changes1.5 or Fold Changes =-1.5) were screened. Omes) and other bioinformatics analysis techniques to enrich the biological functions and pathways of the differential genes. [experimental results] the blood pressure in the blank group remained stable after the model was started. On the third day, the blood pressure of the model group began to rise 120mm Hg, and the blood pressure continued to rise (P0.01) at the end of the day (P0.01). The blood pressure in the acupuncture group was lower than the model group (P0.05) at the beginning of fifth days. The blood pressure in the acupuncture group was not reduced to normal, but the difference between the model group and the model group was statistically significant (P0.05). The genome annotation (Genome annotation) was the use of bioinformatics methods and tools to map all the differential expression genes to the GO number by high flux annotated.GO function enrichment analysis of the biological functions of all genes in the genome. According to each item of the library, the number of differentially expressed genes corresponding to each item was calculated, and the main biological functions of the differentially expressed genes between the two samples were given. The difference of the gene between the electroacupuncture group and the model group and the complex ion channel of the chloride channel complex, the chloride ion binding, the chloride channel activity, and the receptor were found. Activity and other function related.KEGG signaling pathway analysis showed that 7 pathways were activated in the model group. Compared with the model group, 2 pathways were activated in the electroacupuncture group. It was found that the neural receptor pathway (Neuroactive ligand-receptor action) was closely related to hypertension and electroacupuncture, and the GABRB3 gene was expressed in this pathway. According to the analysis of the differentially expressed genes in the electroacupuncture group, GABRB3, CFTR, and CISH genes are often found in the enrichment function and signal pathway. The 3 genes are closely related to the pathogenesis of hypertension through literature retrieval. The mechanism of electroacupuncture decompression may be realized by the function expression and signal transduction pathway involved in the regulation of the 3 key genes. Conclusion]1. corresponds to the intervention of Electroacupuncture of Qu Chi and Tai Chi in prehypertensive rats with induced hypertension. The gene expression profiles of renal tissue in prehypertensive rats with the effect of.2. electroacupuncture and Tai Chung acupoints.3. electroacupuncture Chi Chi and Tai Chung acupoint may be regulated by GABRB3, CFTR, CISH gene and neural receptor activity pathway (Neuroactive). Ligand-receptor action) depressurization.
【學(xué)位授予單位】：北京中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R245

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