本文選題：小擬南芥 + 無苞芥�。� 參考：《植物學(xué)報》2016年03期

【摘要】：鋅指蛋白(ZFP)是一類重要的轉(zhuǎn)錄因子,廣泛參與植物的生長發(fā)育和非生物脅迫應(yīng)答。新疆小擬南芥(Arabidopsis pumila)又名無苞芥,是十字花科短命植物,具有高光效、繁殖力強和適應(yīng)干旱等生物學(xué)特征,而且比模式植物擬南芥(A.thaliana)更耐高鹽脅迫。將前期克隆的小擬南芥鋅指蛋白基因Ap ZFP通過花滴法轉(zhuǎn)化到哥倫比亞生態(tài)型擬南芥(Col-0)中,獲得了獨立表達的轉(zhuǎn)基因株系。表型觀察發(fā)現(xiàn),過量表達Ap ZFP基因可促使擬南芥在長短日照下均提前開花。實時熒光定量PCR結(jié)果顯示,轉(zhuǎn)基因擬南芥株系中,光周期途徑中的CO基因和年齡途徑中的SPL基因表達上調(diào);春化、環(huán)境溫度和自主途徑中的FLC基因表達下調(diào);編碼成花素的基因FT及下游開花相關(guān)基因AP1和LFY的表達量均升高。進一步通過鹽、干旱和ABA脅迫處理Ap ZFP轉(zhuǎn)基因株系的種子和幼苗,發(fā)現(xiàn)在脅迫處理下,與對照相比,轉(zhuǎn)基因擬南芥種子萌發(fā)率較高,幼苗主根較長。因此推測,Ap ZFP在植物發(fā)育過程中具有多種功能,可能既參與植物的開花轉(zhuǎn)變過程,又同其它植物的鋅指蛋白基因一樣,參與植物的耐逆過程。
[Abstract]:Zinc finger protein (ZFP) is an important transcriptional factor which is widely involved in plant growth and abiotic stress response. Xinjiang small Arabidopsis thaliana (Arabidopsis pumila) is a short-lived cruciferous plant with high light efficiency, strong fecundity and drought adaptation, and is more tolerant to salt stress than the model plant, Arabidopsis thaliana (A.thaliana). The previously cloned zinc finger protein gene of Arabidopsis thaliana Ap ZFP was transformed into Colombian ecotypic Arabidopsis thaliana (Col-0) by flower drop method. Phenotypic observation showed that overexpression of Ap ZFP gene promoted the flowering of Arabidopsis thaliana under long and short days. Real-time fluorescence quantitative PCR showed that CO gene expression in photoperiod pathway and SPL gene expression in age pathway were up-regulated in transgenic Arabidopsis thaliana lines, but FLC gene expression was down-regulated in vernalization, ambient temperature and autonomous pathway. The expression of FT and AP1 and LFY genes were increased. The seeds and seedlings of Ap ZFP transgenic lines were further treated with salt, drought and ABA stress. It was found that under stress treatment, the germination rate of transgenic Arabidopsis thaliana seeds was higher and the main root of transgenic Arabidopsis seedlings was longer than that of control. Therefore, it is inferred that Ap ZFP has many functions in plant development, which may not only participate in the process of plant flowering transformation, but also participate in the process of plant stress tolerance, just like the zinc finger protein gene of other plants.
【作者單位】： 石河子大學(xué)生命科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金(No.U1303302,31060149) 石河子大學(xué)高層次人才活動項目(No.RCZX200902)
【分類號】：Q943.2
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本文編號：2082679