發(fā)布時間：2018-06-28 01:56

  本文選題：慢性粒細胞白血病 + BCR-ABL�。� 參考：《檢驗醫(yī)學與臨床》2016年22期

【摘要】：目的探討采用實時熒光定量逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(RQ-PCR)技術(shù)檢測慢性粒細胞白血病(CML)細胞的BCRABLp210融合基因的臨床價值。方法應(yīng)用RQ-PCR技術(shù)對60例CML患者骨髓細胞BCR-ABLp210融合基因進行定量檢測分析。結(jié)果 60例BCR-ABLp210融合基因陽性的CML患者中,慢性期33例,加速期13例,急變期14例,且CML急變期患者BCR-ABLp210轉(zhuǎn)錄本水平明顯高于慢性期和加速期患者。經(jīng)異基因造血干細胞移植治療或經(jīng)伊馬替尼治療6個月后,BCRABLp210轉(zhuǎn)錄本水平均較6個月前明顯降低。但這兩種方法治療CML患者12個月后的效果比較差異無統(tǒng)計學意義(P0.05),而采用其他酪氨酸激酶抑制劑治療CML 12個月后也可達到相似的治療效果。結(jié)論 RQ-PCR技術(shù)監(jiān)測CML患者細胞BCR-ABLp210融合基因表達有助于CML的診斷、治療效果評價及微小殘留的監(jiān)測。
[Abstract]:Objective to investigate the clinical value of real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) in detecting BCR ABL p210 fusion gene in chronic myeloid leukemia (CML) cells. Methods BCR-ABLp210 fusion gene in bone marrow cells of 60 CML patients was quantitatively detected by RQ-PCR. Results among 60 CML patients with positive BCR-ABLp210 fusion gene, 33 cases were in chronic stage, 13 cases in accelerated phase and 14 cases in blast phase. The level of BCR-ABLp210 transcript in CML patients was significantly higher than that in chronic phase and accelerated phase. After 6 months of allogeneic hematopoietic stem cell transplantation or imatinib treatment, the level of BCRABLp210 transcripts was significantly lower than that of 6 months ago. However, there was no significant difference between the two methods after 12 months (P0.05), while other tyrosine kinase inhibitors could achieve similar results after 12 months of CML treatment. Conclusion Detection of BCR-ABLp210 fusion gene expression in CML cells by RQ-PCR is helpful to the diagnosis of CML, evaluation of therapeutic effect and monitoring of minimal residual.
【作者單位】： 武漢大學人民醫(yī)院檢驗科;武漢大學人民醫(yī)院血液內(nèi)科;
【基金】：國家自然科學基金資助項目(81200389,81501427) 教育部高等學校博士學科點專項科研基金(20120141120077) 湖北省自然科學基金資助項目(2016CFB456)
【分類號】：R733.72;R730.43

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