本文選題：慢性粒細(xì)胞白血病 + BCR-ABL�。� 參考：《檢驗(yàn)醫(yī)學(xué)與臨床》2016年22期

【摘要】：目的探討采用實(shí)時(shí)熒光定量逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(RQ-PCR)技術(shù)檢測(cè)慢性粒細(xì)胞白血病(CML)細(xì)胞的BCRABLp210融合基因的臨床價(jià)值。方法應(yīng)用RQ-PCR技術(shù)對(duì)60例CML患者骨髓細(xì)胞BCR-ABLp210融合基因進(jìn)行定量檢測(cè)分析。結(jié)果 60例BCR-ABLp210融合基因陽性的CML患者中,慢性期33例,加速期13例,急變期14例,且CML急變期患者BCR-ABLp210轉(zhuǎn)錄本水平明顯高于慢性期和加速期患者。經(jīng)異基因造血干細(xì)胞移植治療或經(jīng)伊馬替尼治療6個(gè)月后,BCRABLp210轉(zhuǎn)錄本水平均較6個(gè)月前明顯降低。但這兩種方法治療CML患者12個(gè)月后的效果比較差異無統(tǒng)計(jì)學(xué)意義(P0.05),而采用其他酪氨酸激酶抑制劑治療CML 12個(gè)月后也可達(dá)到相似的治療效果。結(jié)論 RQ-PCR技術(shù)監(jiān)測(cè)CML患者細(xì)胞BCR-ABLp210融合基因表達(dá)有助于CML的診斷、治療效果評(píng)價(jià)及微小殘留的監(jiān)測(cè)。
[Abstract]:Objective to investigate the clinical value of real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) in detecting BCR ABL p210 fusion gene in chronic myeloid leukemia (CML) cells. Methods BCR-ABLp210 fusion gene in bone marrow cells of 60 CML patients was quantitatively detected by RQ-PCR. Results among 60 CML patients with positive BCR-ABLp210 fusion gene, 33 cases were in chronic stage, 13 cases in accelerated phase and 14 cases in blast phase. The level of BCR-ABLp210 transcript in CML patients was significantly higher than that in chronic phase and accelerated phase. After 6 months of allogeneic hematopoietic stem cell transplantation or imatinib treatment, the level of BCRABLp210 transcripts was significantly lower than that of 6 months ago. However, there was no significant difference between the two methods after 12 months (P0.05), while other tyrosine kinase inhibitors could achieve similar results after 12 months of CML treatment. Conclusion Detection of BCR-ABLp210 fusion gene expression in CML cells by RQ-PCR is helpful to the diagnosis of CML, evaluation of therapeutic effect and monitoring of minimal residual.
【作者單位】： 武漢大學(xué)人民醫(yī)院檢驗(yàn)科;武漢大學(xué)人民醫(yī)院血液內(nèi)科;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(81200389,81501427) 教育部高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金(20120141120077) 湖北省自然科學(xué)基金資助項(xiàng)目(2016CFB456)
【分類號(hào)】：R733.72;R730.43

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