本文選題：抗病基因同源序列 + RGA法��； 參考：《基因組學(xué)與應(yīng)用生物學(xué)》2017年08期

【摘要】：抗病基因同源序列克隆法即RGA法是克隆抗病基因快速有效的途徑。它是根據(jù)已克隆植物抗病基因的保守結(jié)構(gòu)域設(shè)計簡并引物,擴增獲得RGAs,通過分析RGAs與抗病基因的關(guān)系,確定候選基因,最終獲得抗病基因。目前克隆的RGAs主要是根據(jù)NBS-LRR和STK兩種保守結(jié)構(gòu)域獲得。本綜述介紹了抗病基因的分類、特征及作用,論述了抗病基因同源序列的克隆及其在遺傳圖譜構(gòu)建,基因定位、克隆,植物起源進化及多樣性方面的應(yīng)用及研究進展,為進一步克隆抗病基因、揭示抗病信號途徑及抗病機制奠定基礎(chǔ)。
[Abstract]:RGA method is a rapid and effective way to clone disease resistance genes by homologous sequence cloning. According to the conserved domain of cloned plant disease resistance genes, degenerate primers were designed to amplify and obtain RGAs. by analyzing the relationship between RGAs and disease-resistant genes, the candidate genes were identified, and the disease-resistant genes were finally obtained. At present, the cloned RGAs were obtained from two conserved domains of NBS-LRR and STK. In this paper, the classification, characteristics and functions of resistance genes are reviewed. The cloning of homologous sequences of resistance genes and their applications in genetic map construction, gene mapping, cloning, plant origin, evolution and diversity are reviewed. To further clone disease resistance gene, to reveal the signal pathway of disease resistance and disease resistance mechanism.
【作者單位】： 陜西理工學(xué)院生物科學(xué)與工程學(xué)院;西北農(nóng)林科技大學(xué)農(nóng)學(xué)院;漢臺中學(xué);陜西理工學(xué)院體育學(xué)院;
【基金】：大學(xué)生創(chuàng)新創(chuàng)業(yè)項目(2016110) 陜西省教育廳項目(14JK1152) “春筍計劃”共同資助
【分類號】：Q943.2

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1 賀超英,張志永,陳受宜;大豆中NBS類抗病基因同源序列的分離與鑒定[J];科學(xué)通報;2001年12期

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