本文選題：微小牛蜱 + P基因��； 參考：《黑龍江畜牧獸醫(yī)》2017年09期

【摘要】：為了研究微小牛蜱P0蛋白的免疫原性,試驗采用RT-PCR技術(shù)從微小牛蜱中擴增P0基因,將PCR產(chǎn)物克隆連入PET28a(+)載體構(gòu)建重組表達載體PET28a-P0,再將其轉(zhuǎn)化到大腸桿菌BL21(DE3)感受態(tài)細(xì)胞中以IPTG誘導(dǎo)表達。結(jié)果表明:P0基因獲得表達,表達的重組蛋白分子質(zhì)量為35 ku,表達產(chǎn)物能被微小牛蜱陽性血清所識別。說明P0蛋白具有良好的免疫原性。
[Abstract]:In order to study the immunogenicity of P0 protein of bovine ticks, the P0 gene was amplified from bovine ticks by RT-PCR technology, and PCR product clones were cloned into PET28a (+) vector to construct a recombinant expression vector PET28a-P0, and then transformed into BL21 (DE3) receptive cells in Escherichia coli with IPTG induced expression. The results showed that the P0 gene was expressed and expressed. The recombinant protein has a molecular mass of 35 Ku, and the expressed product can be identified by the positive immunized serum of S. nixus, indicating that P0 protein has good immunogenicity.
【作者單位】： 河北農(nóng)業(yè)大學(xué)動物醫(yī)學(xué)院;阜平縣畜牧水產(chǎn)局;
【基金】：河北省現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系奶牛產(chǎn)業(yè)創(chuàng)新團隊建設(shè)項目(HBCT2013080204) 河北農(nóng)業(yè)大學(xué)科研發(fā)展基金計劃項目(1509003)
【分類號】：S852.746

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