耐弱光基因型馬鈴薯在遮陰條件下的光合和熒光特性分析
發(fā)布時間:2018-06-22 04:35
本文選題:馬鈴薯 + 耐弱光; 參考:《中國生態(tài)農(nóng)業(yè)學報》2017年08期
【摘要】:為探尋遮陰對馬鈴薯光合和熒光特性及吸收光能分配的影響,本研究采用大田試驗,以馬鈴薯不耐弱光品種‘麗薯6號’和耐弱光品種‘中薯20’為材料,出苗后用遮光率70%的黑色遮陽網(wǎng)進行遮陰處理(T),以自然光照為對照(CK),測定了遮陰30 d后馬鈴薯葉片的光合作用、光響應(yīng)曲線、CO_2響應(yīng)曲線、光誘導曲線和葉綠素熒光參數(shù)的變化。結(jié)果表明:1)遮陰后凈光合速率(P_n)、氣孔導度(G_s)、蒸騰速率(T_r)、最大凈光合速率(P_(max))、光飽和點(LSP)、光補償點(LCP)較CK顯著下降;‘中薯20’的P_n、P_(max)、LSP較高,LCP和暗呼吸速率(R_d)較低。2)不同基因型CO_2響應(yīng)參數(shù)無顯著差異,但‘中薯20’的最大羧化速率(V_(cmax))和最大電子傳遞速率(Jmax)較高,CO_2補償點(CCP)較低。3)高光誘導過程中,‘中薯20’反應(yīng)較快,光合能力較強。4)初始熒光(F_o)、最大熒光(F_m)、最大光化學量子效率(F_v/F_m)較CK顯著增加,PSⅡ?qū)嶋H光化學量子效率(?F/F_m′)、表觀電子傳遞速率(ETR)和光化學猝滅系數(shù)(qP)較CK顯著下降,非光化學猝滅系數(shù)(NPQ)呈增加趨勢。5)非光化學熱耗散(ФNPQ)和熒光耗散途徑(Фf,d)比例顯著增加,光化學猝滅耗散途徑(ФPSⅡ)比例顯著減少,主要以增加熱耗散為主。遮陰后,耐弱光基因型‘中薯20’的NPQ和ФNPQ均高于不耐弱光的‘麗薯6號’,說明‘中薯20’的光合機構(gòu)保護能力更強。綜合分析表明,遮陰后耐弱光基因型馬鈴薯具有較高的凈光合速率、較低的光補償點、較低的CO_2補償點、較快的光誘導反應(yīng)速度和較高的非光化學熱耗散能力。
[Abstract]:In order to explore the effect of shading on photosynthetic and fluorescence characteristics and absorption energy distribution of potato, the field experiment was carried out in this paper. The potato varieties' Lishu 6 'and' Zhongshu 20 'were used as the materials, and the two varieties,' Lishu 6 'and' Zhongshu 20', were used as materials. After seedling emergence, shading treatment (T) was carried out with a black sunshade net with 70% shading rate. The photosynthesis, photoresponse curve, photo-induced curve and chlorophyll fluorescence parameters of potato leaves after 30 days shading were measured by using natural light as control (CK). The results showed that net photosynthetic rate (PN), stomatal conductance, transpiration rate, maximum net photosynthetic rate (P _ (max), light saturation point) and light compensation point (LCP) were significantly decreased after shading. There was no significant difference in CO2 response parameters among different genotypes. However, the maximum carboxylation rate (V _ (cmax) and maximum electron transfer rate (Jmax) of 'Zhongshu 20' was higher than that of Cost2 compensation point (CCP). 3) in the process of high light induction, the reaction of 'Zhongshu 20' was faster. Compared with CK, the maximum photochemical quantum efficiency (FV / FM), the apparent electron transfer rate (ETR) and the photochemical quenching coefficient (QP) of PS 鈪,
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