本文選題：羽衣甘藍(lán) + 擬南芥��； 參考：《南方農(nóng)業(yè)學(xué)報(bào)》2017年05期

【摘要】：【目的】分析轉(zhuǎn)擬南芥△1-吡咯啉-5-羧酸合成酶(P5CS1)基因羽衣甘藍(lán)的耐鹽性,為獲得較強(qiáng)的耐鹽性羽衣甘藍(lán)品種及其抗逆育種提供理論依據(jù)�！痉椒ā繉M南芥P5CS1基因(AtP5CS1)經(jīng)農(nóng)桿菌介導(dǎo)轉(zhuǎn)入羽衣甘藍(lán)植物中,在鹽脅迫下,分別檢測轉(zhuǎn)基因植株與野生型植株的AtP5CS1 mRNA表達(dá)量、幼苗脯氨酸含量、株系根系性狀、整株干質(zhì)量和鮮質(zhì)量、葉片相對水含量、葉片電導(dǎo)率和整株存活率�！窘Y(jié)果】在150 mmol/L NaCl脅迫下,轉(zhuǎn)基因植株的P5CS1基因mRNA可正常表達(dá),與對照相比,轉(zhuǎn)基因株系Y1、Y2的主根和最長側(cè)根長度較長,側(cè)根數(shù)目較多,整株干質(zhì)量和鮮質(zhì)量較重;而且相對水含量顯著高于對照植株(P0.05,下同),脯氨酸含量及存活率均極顯著高于對照植株(P0.01),葉片相對電導(dǎo)率顯著低于對照植株�！窘Y(jié)論】轉(zhuǎn)AtP5CS1基因植株的耐鹽表型優(yōu)于對照,即AtP5CS1基因在羽衣甘藍(lán)中的表達(dá)明顯改善了轉(zhuǎn)基因植株的耐鹽性。
[Abstract]:[objective] to study the salt-tolerance of Brassica oleracea, a transgenic Arabidopsis thaliana 1-pyrrolidin-5-carboxylate synthase (P5CS1) gene. [methods] Arabidopsis thaliana P5CS1 gene (AtP5CS1) was transferred into Brassica oleracea by Agrobacterium tumefaciens. AtP5CS1 mRNA expression, proline content, root characters, dry and fresh weight of whole plant and relative water content of leaves were detected in transgenic and wild type plants, respectively. [results] under 150 mmol / L NaCl stress, the expression of P5CS1 mRNA in transgenic plants was normal. Compared with the control, the length of the main root and the longest lateral root of the transgenic strain Y1C-1 Y2 was longer, and the number of lateral roots was more. The dry and fresh weight of the whole plant was heavier; The relative water content was significantly higher than that of the control plants (P0.05, the same below), the proline content and survival rate were significantly higher than those of the control plants (P0.01), and the relative electrical conductivity of the leaves was significantly lower than that of the control plants. [conclusion] the salt-tolerant phenotype of the transgenic plants with AtP5CS1 gene was superior to that of the control plants. That is, the expression of AtP5CS1 gene in Brassica oleracea significantly improved the salt tolerance of transgenic plants.
【作者單位】： 黃淮學(xué)院生物與食品工程學(xué)院;駐馬店市綠化處;駐馬店市南海公園;駐馬店市園林綠化科研所;
【基金】：河南省自然科學(xué)基金項(xiàng)目(112300410042) 河南省科技發(fā)展計(jì)劃項(xiàng)目(172102410086) 河南省教育廳高校重點(diǎn)項(xiàng)目(16A210032)
【分類號】：S635.9

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1 李鴻雁;李大紅;;轉(zhuǎn)擬南芥P5CS1基因增強(qiáng)羽衣甘藍(lán)的耐旱性[J];植物生理學(xué)報(bào);2014年07期

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