本文選題：藏羊 + 冷季補飼�。� 參考：《畜牧獸醫(yī)學報》2017年02期

【摘要】：本試驗旨在研究冷季補飼精料或尿素-糖蜜型舔磚對藏羊小腸組織形態(tài)發(fā)育及營養(yǎng)物質轉運載體基因表達量的影響。選擇1.5歲、體重((29.4±1.79)kg)相近的健康藏系綿羊母羊18頭,隨機分為對照組(CON組)、尿素-糖蜜型舔磚補飼組(BS組)和精料補飼組(CS組)。CON組自由采食燕麥干草,BS組在CON組基礎上自由舔食尿素-糖蜜型舔磚,CS組在CON組基礎上補飼精料200g·只-1·d-1。進行60d的飼養(yǎng)試驗后,對全部試驗藏羊進行屠宰,采集小腸各段組織,通過制作組織切片及熒光定量PCR,測定小腸組織形態(tài)發(fā)育和營養(yǎng)物質轉運載體基因表達量。結果表明:(1)補飼精料或舔磚均顯著提高了藏羊消化能和粗蛋白質攝入量(P0.05),且CS組顯著高于BS組(P0.05);(2)補飼精料或舔磚均顯著提高了藏羊十二指腸、空腸及回腸絨毛寬度(P0.05),且CS組均顯著大于BS組(P0.05),補飼精料還顯著提高了十二指腸和回腸絨毛高度(P0.05),顯著降低了空腸和回腸隱窩深度(P0.05);(3)胰島素樣生長因子結合蛋白5(Insulin-like growth factor binding protein,IGFBP5)mRNA表達量在十二指腸和空腸黏膜中CS和BS組均顯著高于CON組(P0.05),且CS組顯著高于BS組(P0.05),在回腸黏膜中CS組顯著高于BS和CON組(P0.05);(4)十二指腸黏膜中L型氨基酸轉運載體1(L-type amino acid transporter 1,LAT1)、陽離子氨基酸轉運載體1(Cationic amino acid transporters 1,CAT1)、小肽轉運載體1(Peptides transporter 1,pepT1)、鈉依賴型葡萄糖轉運載體1(Na+-dependent glucose co-transporter 1,SGLT1)及促葡萄糖轉運載體2(Facilitative glucose transporter 2,GLUT2)mRNA表達量CS組顯著高于BS和CON組(P0.05),空腸黏膜中CAT1、pepT1mRNA表達量和回腸黏膜中CAT1、LAT1、SGLT1及GLUT2mRNA表達量,BS組顯著高于CS和CON組(P0.05)。以上結果表明,冷季補飼精料或尿素-糖蜜型舔磚均增加了藏羊對能量和蛋白質等營養(yǎng)物質的攝入量,促進了小腸組織形態(tài)發(fā)育,補飼精料提高了十二指腸黏膜中氨基酸、小肽及葡萄糖轉運載體基因mRNA的表達量,而補飼尿素-糖蜜型舔磚提高了其在空腸和回腸黏膜中的表達量。
[Abstract]:The purpose of this study was to study the effects of supplementation of concentrate or urea-molasses licking brick on intestinal tissue morphology and gene expression of nutrient transport vector in Tibetan sheep. 18 healthy Tibetan sheep, aged 1.5 years and weighing 29.4 鹵1.79 kg, were selected. The rats were randomly divided into control group (Con group), urea-molasses type licking brick supplement group (BS group) and concentrate supplement group (CS group). Con group fed freely oat hay on the basis of Con group and CS group fed 200 g of concentrate on the basis of Con group. After 60 days of feeding experiment, all the Tibetan sheep were slaughtered, and the tissues of small intestine were collected. By making tissue sections and fluorescence quantitative PCR, the expression of small intestine tissue morphology and nutrient transport vector gene was determined. The results showed that the dietary energy and crude protein intake of Tibetan sheep were significantly increased by supplementing the concentrate or licking brick, and that in CS group was significantly higher than that in BS group (P 0.05). The supplementary feeding concentrate or licking brick significantly increased the duodenum of Tibetan sheep. The width of jejunum and ileum villi was significantly larger in CS group than that in BS group. Supplementation of concentrate significantly increased the height of duodenum and ileum villi, and significantly decreased the depth of jejunum and ileum crypt (P0.05) insulin-like growth factor-binding protein (IGF- binding protein). The expression of IGFBP5 mRNA in duodenal and jejunal mucosa was significantly higher in CS and BS group than that in Con group, and in CS group was significantly higher than that in BS group, and in CS group was significantly higher than that in BS group and Con group. The expression levels of L-type amino acid transporter 1, cationic amino acid transporters 1, Peptides transporter 1 pepT1, sodium dependent glucose transport vector 1Na-dependent glucose co-transporter 1G LT1 and glucose stimulating glucose transporter 2G UT2 mRNA expression in CS group were significantly higher than those in control group (P < 0. 05), while the expression of cationic amino acid transport vector was 1 ~ (-1), Cationic amino acid transporters _ (1) and small peptide transport vector 1 ~ (1) Peptides transporter _ (1) peptid T _ (1). The expression of CAT1pepT1 mRNA in jejunum and ileum mucosa was significantly higher in BS and Con groups than that in CS and Con groups. The results indicated that supplemental feeding of concentrate or urea-molasses type licking brick in cold season increased the intake of nutrients such as energy and protein in Tibetan sheep, promoted the development of small intestine tissue, and increased the amino acids in duodenal mucosa by supplementary feeding concentrate. The mRNA expression of small peptide and glucose transport vector gene was increased by supplementation of urea-molasses licking brick. The expression of small peptide and glucose transport vector gene in jejunum and ileum mucosa was increased by supplementation of urea-molasses type lick brick.
【作者單位】： 四川農業(yè)大學動物營養(yǎng)研究所;海北高原現(xiàn)代生態(tài)畜牧業(yè)科技試驗示范園;
【基金】：青藏高原社區(qū)畜牧業(yè)行業(yè)科技項目(201203008)
【分類號】：S826.83

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