本文選題：轉(zhuǎn)基因大米 + 差異蛋白質(zhì)組學��； 參考：《國際藥學研究雜志》2016年04期

【摘要】：目的利用差異蛋白質(zhì)組學及生物信息學等技術(shù)方法,探討外源Bt基因的導入對大米表達蛋白質(zhì)組的影響,深化轉(zhuǎn)基因大米在蛋白質(zhì)組學方面的研究。方法在轉(zhuǎn)基因大米"華恢1號"和親本大米"明恢63"樣品中提取大米總蛋白,通過二維聚丙烯酰胺凝膠電泳(2D-PAGE)實驗方法,得到相應(yīng)的蛋白質(zhì)組2D-PAGE譜,再選擇差異明顯的蛋白質(zhì)點進行質(zhì)譜鑒定及生物信息學分析。結(jié)果對轉(zhuǎn)Bt(cry1Ab/1Ac)基因大米和親本大米2D-PAGE圖譜的蛋白質(zhì)點進行了對比匹配,識別出明顯的差異蛋白質(zhì)點28個,以親本大米為參照,轉(zhuǎn)Bt基因大米相對高表達的18個,相對低表達的10個;選擇轉(zhuǎn)基因大米凝膠上的差異蛋白質(zhì)點進行了質(zhì)譜鑒定和生物信息學檢索,發(fā)現(xiàn)差異蛋白質(zhì)主要參與能量代謝,蛋白質(zhì)合成、氧化還原和應(yīng)激響應(yīng)等生物過程。結(jié)論轉(zhuǎn)Bt基因"華恢1號"及其親本大米"明恢63"表達的蛋白質(zhì)組存在一定差異,但未發(fā)現(xiàn)這些差異蛋白質(zhì)具有抗營養(yǎng)性和致敏性,也未發(fā)現(xiàn)新蛋白和毒蛋白的表達。
[Abstract]:Objective to investigate the effect of exogenous BT gene introduction on the expression of proteome in rice by using differential proteomics and bioinformatics, and to deepen the proteomics of transgenic rice. Methods Total protein of rice was extracted from transgenic rice "Huanghui 1" and parent rice "Minghui 63", and the corresponding proteome 2D-PAGE was obtained by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Protein spots with obvious difference were selected for mass spectrometry identification and bioinformatics analysis. Results the protein spots in the 2D-PAGE map of transgenic BT cry1Ab / 1Acc gene rice and parental rice were compared and matched. 28 distinct differentially expressed protein spots were identified, 18 of which were relatively overexpressed in transgenic BT rice, compared with parental rice. The differential protein spots on the gels of transgenic rice were identified by mass spectrometry and bioinformatics search. It was found that differential proteins were mainly involved in energy metabolism and protein synthesis. Biological processes such as redox and stress response. Conclusion the proteome of transgenic BT gene "Huanghui 1" and its parent rice "Minghui 63" has some differences, but these proteins have not been found to be nutritionally resistant and sensitized, nor have the expression of new protein and toxin protein been found.
【作者單位】： 國家生物醫(yī)學分析中心;
【基金】：轉(zhuǎn)基因生物新品種培育國家科技重大專項資助項目(2014ZX08011007) 國家重大科學儀器設(shè)備開發(fā)專項資助項目(2012YQ180117)
【分類號】：S511

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