發(fā)布時(shí)間：2018-06-19 00:42

  本文選題：蝴蝶蘭 + 低溫脅迫��； 參考：《基因組學(xué)與應(yīng)用生物學(xué)》2017年06期

【摘要】：為研究蝴蝶蘭Rubisco活化酶(RCA)基因?qū)Φ蜏孛{迫的響應(yīng)機(jī)制,以蝴蝶蘭"滿天紅"為材料,分析了蝴蝶蘭Rubisco活化酶基因PhRCAβ的全長序列(Gen Bank登錄號(hào)為KU954548)及其表達(dá)特性。結(jié)果表明,PhRCAβ基因全長1 695 bp,編碼440個(gè)氨基酸;其編碼的蛋白具有RCA的特異位點(diǎn),含葉綠體轉(zhuǎn)運(yùn)肽,定位于葉綠體中,其成熟蛋白的分子量為42.52 k D,等電點(diǎn)為蛋白5.54,屬于RCA小亞基基因;該基因在綠色組織中轉(zhuǎn)錄表達(dá);13℃/8℃(晝/夜)的低溫脅迫抑制Ph RCAβ基因的轉(zhuǎn)錄表達(dá),并隨著低溫脅迫時(shí)間的延長,PhRCAβ基因的表達(dá)水平逐漸降低,在溫度恢復(fù)正常時(shí)其表達(dá)水平升高;4℃低溫條件下,Ph RCAβ基因的表達(dá)水平隨著低溫處理時(shí)間的延長逐漸降低,并一直維持在較低水平。由此推測,PhRCAβ直接參與了蝴蝶蘭葉片光合作用的調(diào)控。
[Abstract]:In order to study the response mechanism of Rubisco activase (RCA) gene of Phalaenopsis to low temperature stress, the full-length sequence of Rubisco activase gene PhRCA 尾 of Phalaenopsis, named KU954548, was analyzed. The results showed that the full length of PhRCA 尾 gene was 1 695 BP, encoding 440 amino acids, and the encoded protein had the specific site of RCA, which contained chloroplast transporter peptide and was located in chloroplast. The molecular weight of the mature protein is 42.52 KD, the isoelectric point is 5.54, which belongs to the RCA small subunit gene, and the low temperature stress of 13 鈩，

本文編號(hào)：2037546