本文選題：細(xì)胞增殖抑制基因 + 慢性阻塞性肺疾病��； 參考：《中華中醫(yī)藥雜志》2017年03期

【摘要】：目的:探索慢性阻塞性肺疾病急性加重期(AECOPD)病程中細(xì)胞增殖抑制基因(HSG)與AECOPD致病因素吸煙的相關(guān)性,為進(jìn)一步認(rèn)識(shí)AECOPD發(fā)病本質(zhì)創(chuàng)造條件。方法:隨機(jī)選取80例AECOPD患者及30名正常人為研究對(duì)象,分為吸煙組、非吸煙組、正常組。所有入選者空腹抽取靜態(tài)血液,用試劑盒法提取血液總RNA,用Real-time PCR檢測(cè)各組AECOPD患者血清中HSG mRNA的表達(dá);采用Western bolt檢測(cè)各組AECOPD患者血清中HSG蛋白的表達(dá)。結(jié)果:與正常組比較,吸煙組及非吸煙組HSG mRNA及HSG蛋白的表達(dá)均降低(P0.05)。結(jié)論:HSG在AECOPD患者血液中低表達(dá),在正常組血液中高表達(dá);吸煙因素可能通過影響HSG的表達(dá)而影響了AECOPD氣道重建。
[Abstract]:Objective: to explore the relationship between HSGG and smoking in AECOPD patients with acute exacerbation of chronic obstructive pulmonary disease (COPD), so as to create conditions for further understanding the nature of AECOPD. Methods: 80 AECOPD patients and 30 normal subjects were randomly divided into smoking group, non smoking group and normal group. All the subjects were selected to extract static blood on an empty stomach, total RNAs were extracted by kit method, the expression of HSG mRNA in serum of AECOPD patients was detected by Real-time PCR, and the expression of HSG protein in serum of AECOPD patients was detected by Western bolt. Results: compared with the normal group, the expression of HSG mRNA and HSG protein in smoking group and non smoking group decreased significantly (P 0.05). Conclusion the expression of HSG is low in AECOPD patients and high in normal blood, and smoking may affect the airway remodeling of AECOPD by affecting the expression of HSG.
【作者單位】： 貴陽中醫(yī)學(xué)院;貴陽中醫(yī)學(xué)院第二附屬醫(yī)院呼吸內(nèi)科;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(No.81473533,No.81260599) 貴州省長(zhǎng)基金項(xiàng)目(No.黔省專合字[2011]62號(hào)) 貴州省攻關(guān)課題(No.黔科合SY[2010]3106號(hào))~~
【分類號(hào)】：R563.9

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