本文選題：小黑楊 + 基因表達(dá)��； 參考：《東北林業(yè)大學(xué)學(xué)報(bào)》2017年08期

【摘要】：從小黑楊中克隆915 bp的NAC7轉(zhuǎn)錄因子基因(Potri.007G099400.1)cDNA,其編碼304氨基酸,該蛋白屬熱穩(wěn)定性較高的親水性蛋白。用RT-qPCR分析楊樹(shù)鹽脅迫條件下NAC7基因表達(dá)情況,表明該基因?qū)}脅迫具有應(yīng)答反應(yīng),且基因主要在根部表達(dá)�？寺～@得1 062 bp的NAC7基因啟動(dòng)子DNA序列,其中含有許多脅迫應(yīng)答元件,其驅(qū)動(dòng)的GUS報(bào)告基因主要集中在根中表達(dá),而在莖和葉中的表達(dá)很少,這個(gè)結(jié)果與NAC7基因表達(dá)進(jìn)行RT-qPCR分析結(jié)果一致。
[Abstract]:A 915bp NAC7 transcription factor gene, Potri.007G099400.1, was cloned from Populus nigra. It encodes 304 amino acids and belongs to a hydrophilic protein with high thermal stability. The expression of NAC7 gene in poplar under salt stress was analyzed by RT-qPCR. The results showed that NAC7 gene was responsive to salt stress and mainly expressed in roots. A 1 062 BP DNA sequence of the promoter of NAC7 gene was obtained, which contained many stress response elements. The Gus reporter gene driven by NAC7 gene was mainly expressed in roots, but not in stems and leaves. The results were consistent with the results of RT-qPCR analysis of NAC7 gene expression.
【作者單位】： 林木遺傳育種國(guó)家重點(diǎn)實(shí)驗(yàn)室(東北林業(yè)大學(xué));
【基金】：國(guó)家“863”課題(2013AA102701)
【分類號(hào)】：Q943.2;S792.11

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