發(fā)布時(shí)間：2018-06-18 05:11

  本文選題：巨型住肉孢子蟲(chóng) + 藏羊��； 參考：《中國(guó)獸醫(yī)雜志》2017年10期

【摘要】：為了研究青海省巨型住肉孢子蟲(chóng)線粒體細(xì)胞色素C氧化酶第一亞基(cox1)基因部分序列(pcox1)的遺傳變異情況,并用pcox1序列構(gòu)建巨型住肉孢子蟲(chóng)與其他住肉孢子蟲(chóng)的種群遺傳關(guān)系。利用PCR技術(shù)擴(kuò)增巨型住肉孢子蟲(chóng)的pcox1,將測(cè)序所獲序列進(jìn)行比對(duì),并進(jìn)行同源性分析和系統(tǒng)發(fā)育樹(shù)的構(gòu)建。結(jié)果顯示,所獲得的pcox1序列長(zhǎng)度均為968 bp,與GenBank已公布的巨型住肉孢子蟲(chóng)相似度為99.1%~99.8%。系統(tǒng)發(fā)育樹(shù)表明,所有分離株與已知巨型住肉孢子蟲(chóng)位于同一分支。巨型住肉孢子蟲(chóng)pcox1序列種內(nèi)相對(duì)保守(0.2%~0.7%),種間差異較大(6.8%~20.8%),可用作種間遺傳變異研究的標(biāo)記。
[Abstract]:In order to study the genetic variation of the mitochondrial cytochrome C oxidase subunit 1 (cox1) gene of megasporidium in Qinghai province, the population genetic relationship between megasporidium and other megasporidium was constructed by using pcox1 sequence. The PCR technique was used to amplify the pcox1 of megasporidium. The sequence obtained by sequencing was compared and the homology analysis and phylogenetic tree construction were carried out. The results showed that the length of the obtained pcox1 sequences was 968 BP, and the similarity between the pcox1 sequences and the meatosporidium published in GenBank was 99.1 and 99.8, respectively. Phylogenetic tree showed that all isolates were located in the same branch as the known meatosporidium. The pcox1 sequence of megasporidium was relatively conserved within the species and 0.20.70%, and the difference between species was relatively large (6.8% and 20.8%), which could be used as a marker for the study of interspecific genetic variation.
【作者單位】： 青海大學(xué)農(nóng)牧學(xué)院;青海省畜牧獸醫(yī)科學(xué)院;
【基金】：青海省農(nóng)牧廳科技專(zhuān)項(xiàng)(NMSY-2015-01) 國(guó)家自然科學(xué)基金(31660698)
【分類(lèi)號(hào)】：S852.723

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