本文選題：海藻糖 + 酶法制備��； 參考：《食品科技》2017年08期

【摘要】：海藻糖分子是由2個(gè)吡喃葡萄糖環(huán)以α,α-1,1-糖苷鍵連接而成的一種十分穩(wěn)定的非還原二糖,由于其特殊的生物學(xué)保護(hù)功用,廣泛應(yīng)用于醫(yī)藥、化妝品、食品等多個(gè)領(lǐng)域,具有很好的市場前景。目前,海藻糖的生產(chǎn)方法以生物酶法制備為主。其中,利用Tre Y-Tre Z途徑由淀粉生產(chǎn)海藻糖和利用Tre S途徑由麥芽糖生產(chǎn)海藻糖最為經(jīng)濟(jì)可行。針對(duì)谷氨酸棒桿菌ATCC14067的海藻糖合酶基因(tre S)進(jìn)行基因克隆,將其插入表達(dá)質(zhì)粒p ET-22b,在宿主菌E.coli BL21(DE3)中進(jìn)行誘導(dǎo)表達(dá),成功得到特異表達(dá)的目的蛋白。經(jīng)高效液相色譜檢測證實(shí),該重組酶作用于麥芽糖后,可以產(chǎn)生海藻糖,具有海藻糖合酶的相應(yīng)活性。在最適反應(yīng)條件(30℃,p H7.5)下海藻糖合酶的轉(zhuǎn)化效率可達(dá)60%~70%。實(shí)驗(yàn)系統(tǒng)地對(duì)谷氨酸棒桿菌ATCC14067的海藻糖合酶基因進(jìn)行了異源表達(dá)及酶學(xué)性質(zhì)的研究,為其大規(guī)模生產(chǎn)及工業(yè)化應(yīng)用奠定了基礎(chǔ)。
[Abstract]:Trehalose molecule is a kind of stable non-reducing disaccharide which is composed of two glucopyranosaccharide rings connected by 偽, 偽 -1ta-1-glucoside bond. Due to its special biological protective function, trehalose is widely used in many fields, such as medicine, cosmetics, food, etc. There is a good market prospect. At present, trehalose is mainly produced by biological enzyme method. Among them, trehalose production from starch by Tre Y-Tre Z pathway and trehalose from maltose by Tre S pathway is the most economical and feasible. The trehalose synthase gene of Corynebacterium glutamicum ATCC14067 was cloned and inserted into the expression plasmid pET-22b. High performance liquid chromatography (HPLC) showed that the recombinant enzyme could produce trehalose and had the corresponding activity of trehalose synthase after acting on maltose. The conversion efficiency of trehalose synthase reached 60% under the optimum reaction conditions (30 鈩，

本文編號(hào)：2030466