本文選題：牦牛 + FKBP��； 參考：《畜牧獸醫(yī)學報》2017年01期

【摘要】：本研究旨在了解牦牛FKBP6基因5′調控區(qū)和啟動子區(qū)特征,為探討牦牛和犏牛睪丸組織中FKBP6基因差異表達機制提供依據(jù)。利用克隆測序獲得牦牛FKBP6基因5′調控區(qū)序列,采用生物信息學方法分析其序列特征;采用雙熒光素酶報告基因系統(tǒng)鑒定牦牛FKBP6基因核心啟動子區(qū),利用生物信息學軟件預測與精子發(fā)生有關的轉錄因子結合位點。通過克隆測序和序列拼接獲得了1 354bp的牦牛FKBP6基因5′調控區(qū)序列,與普通牛的一致性為99.71%;牦牛FKBP6基因5′調控區(qū)序列含有潛在的啟動子區(qū)、典型的CAAT-Box和CpG島,但未見TATA-Box;熒光素酶活性分析發(fā)現(xiàn),牦牛FKBP6基因的核心啟動子區(qū)位于5′調控區(qū)的-263~-167nt區(qū)域,含有CAAT-Box、E-Box、CTCF和CREB等與精子發(fā)生相關的轉錄因子結合位點。牦牛FKBP6基因核心啟動子的鑒定和精子發(fā)生相關轉錄因子結合位點的發(fā)現(xiàn)為進一步研究牦牛睪丸組織中FKBP6基因的表達調控奠定了基礎。
[Abstract]:The aim of this study was to understand the characteristics of the 5 'regulatory region and promoter region of FKBP6 gene in yak and to provide evidence for the differential expression mechanism of FKBP6 gene in testis. The sequence of 5 'regulatory region of FKBP6 gene was obtained by cloning and sequencing, and the sequence characteristics of FKBP6 gene were analyzed by bioinformatics, and the core promoter region of FKBP6 gene was identified by double luciferase reporter gene system. Bioinformatics software was used to predict transcription factor binding sites related to spermatogenesis. The sequence of 5 '-regulatory region of 1 354bp FKBP6 gene was obtained by cloning and sequencing, and the sequence was 99.71, and the sequence of 5' -regulatory region of FKBP6 gene of yak contained potential promoter region, typical CAAT-Box and CpG island, the sequence of 5 '-regulatory region of FKBP6 gene of yak was found to be 99.71, and the sequence of 5' regulatory region of FKBP6 gene of yak contained potential promoter region. However, TATA-Box. luciferase activity analysis showed that the core promoter of FKBP6 gene was located in -263 ~ 167nt region of 5'regulatory region, which contained CAAT-BoxCF-BoxP6 CTCF and CREB transcription factor binding sites related to spermatogenesis. The identification of the core promoter of the FKBP6 gene and the discovery of spermatogenetic transcription factor binding sites in yak laid a foundation for further study on the expression and regulation of FKBP6 gene in yak testis.
【作者單位】： 南京農業(yè)大學動物科技學院;
【基金】：國家自然科學基金(30500360)
【分類號】：S823.85

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