本文選題：白茶 + 萎凋��； 參考：《南方農(nóng)業(yè)學(xué)報》2016年08期

【摘要】：【目的】研究白茶萎凋過程中茶多酚、兒茶素組分含量變化與其合成途徑中關(guān)鍵酶基因的動態(tài)表達,為優(yōu)化白茶的萎凋工藝提供參考依據(jù)。【方法】以不同萎凋階段的白茶為原料,分別采用福林酚(Folin-Ciocalten)比色法和高效液相色譜法(HPLC)測定茶多酚、兒茶素組分含量的變化,并以實時熒光定量PCR檢測白茶萎凋過程中與兒茶素物質(zhì)代謝合成相關(guān)基因(PAL、C4H、CHS、CHI、F3'5'H、F3'H、F3H、DFR、LAR、ANS、ANR和UGGT)的相對表達量。【結(jié)果】白茶萎凋過程中茶多酚含量呈逐漸降低趨勢,兒茶素組分中表兒茶素(EC)、沒食子兒茶素(GC)、表沒食子兒茶素(EGC)、表兒茶素沒食子酸酯(ECG)含量整體上呈先上升后下降的變化趨勢,且均在萎凋歷時32 h出現(xiàn)最大值。實時熒光定量PCR檢測結(jié)果表明,兒茶素生物合成途徑關(guān)鍵酶基因PAL、C4H、CHI、F3H、F3'H、F3'5'H、DFR、LAR、ANR和UGGT均在萎凋歷時32 h呈上調(diào)表達,CHS基因則隨萎凋歷時的增加呈下調(diào)表達趨勢,ANS基因表達在整個萎凋過程中呈先上升后下降的變化趨勢,在萎凋歷時16 h達最大值。白茶萎凋過程中兒茶素合成途徑關(guān)鍵基因表達水平具有調(diào)控兒茶素生物合成的作用�！窘Y(jié)論】在白茶萎凋過程中,兒茶素生物合成途徑關(guān)鍵酶基因PAL、C4H、F3H、F3'H、DFR、LAR、ANR的表達與兒茶素組分單體EC、GC、EGC的積累規(guī)律基本一致,其最大值均出現(xiàn)在萎凋歷時32 h,因此在制茶時可適當縮短萎凋時長,以提高白茶品質(zhì)。
[Abstract]:[objective] to study the changes of tea polyphenols and catechins during withering and the dynamic expression of key enzyme genes in its synthesis pathway. In order to optimize the withering process of white tea, the contents of polyphenols and catechins were determined by Folin-Ciocaltenin colorimetric method and HPLC- HPLC. [methods] the content of polyphenols and catechins in tea was determined by Folin-Ciocaltenin colorimetry with different withering stages, [methods] the content of tea polyphenols and catechins was determined by HPLC. Real-time quantitative PCR was used to detect the relative expression levels of the genes associated with catechin metabolism and synthesis in white tea during withering. The content of tea polyphenols decreased gradually during withering of white tea during withering. The contents of epigallocatechin (ECG), non-catechin (GCN), epigallocatechin (EGCN) and epigallocatechin gallate (ECG) in the components of catechins increased first and then decreased, and all of them showed the maximum value at 32 h after withering. The results of real-time fluorescence quantitative PCR showed that, The key enzyme gene of catechin biosynthesis pathway, PALX C4HN, F3H, F3, F3, F3, F3, F3, F3, F3, F3, F5, and UGGT, were up-regulated and up-expressed during withering for 32 h, but down-regulated with the increase of withering duration, and the expression of ans gene increased first and then decreased during the whole process of withering, and then decreased in the whole course of withering, and the expression of CHS gene was down-regulated with the increase of withering time, and the expression of ANR and UGGT were down-regulated. The maximum withering time was 16 h. The key gene expression level of catechin biosynthesis pathway plays an important role in the regulation of catechin biosynthesis. [conclusion] during the withering process of white tea, catechin biosynthesis can be regulated. The expression of the key enzyme gene of catechin biosynthesis pathway, PALX C4HN, F3H, F3H, DFRRNR, was basically consistent with the accumulation rule of catechin component monomer ECG C, and its maximum value appeared in the withering time of 32 h. Therefore, the withering time could be shortened properly in tea making in order to improve the quality of white tea.
【作者單位】： 福建農(nóng)林大學(xué)園藝學(xué)院/茶學(xué)福建省高等學(xué)校重點實驗室;
【基金】：國家自然科學(xué)基金項目(31270735) 福建茶產(chǎn)業(yè)農(nóng)技推廣服務(wù)試點建設(shè)項目(KNJ-151001)
【分類號】：TS272.5

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本文編號：2022283