本文選題：陸地棉 + 逆境脅迫��； 參考：《作物學(xué)報(bào)》2017年10期

【摘要】：SRO是植物特有的類RCD-ONE蛋白家族,在植物抵御各種生物和非生物脅迫過程中具有重要的作用。本研究利用生物信息學(xué)方法從陸地棉遺傳標(biāo)準(zhǔn)系TM-1(Gossypium hirsutum L.acc.TM-1)基因組中鑒定到12個(gè)SRO基因家族成員。多重序列比對(duì)及進(jìn)化樹分析表明,12個(gè)陸地棉SRO均含有PARP和RST結(jié)構(gòu)域,聚為3類,即A、B和C亞家族。轉(zhuǎn)錄組數(shù)據(jù)分析表明,TM-1全基因組中的Gh_D12G1442在莖中優(yōu)勢(shì)表達(dá),Gh_D05G2064在萼片和雄蕊中優(yōu)勢(shì)表達(dá);Gh_A05G3788在雄蕊中優(yōu)勢(shì)表達(dá),Gh_A12G1318在雄蕊和纖維中優(yōu)勢(shì)表達(dá);Gh_A12G2480、Gh_D12G2608、Gh_A05G2257和Gh_D05G2516在纖維中優(yōu)勢(shì)表達(dá);Gh_A08G1390、Gh_D08G1685、Gh_A12G2663和Gh_D12G2054具有較為廣泛的組織表達(dá)。逆境脅迫處理試驗(yàn)表明,Gh_A08G1390明顯受冷處理和鹽處理的誘導(dǎo),Gh_D12G2054、Gh_D08G1685、Gh_A12G2663基因在不同的脅迫處理下受到不同程度的誘導(dǎo)。
[Abstract]:SRO is a plant specific RCD-ONE protein family, which plays an important role in plant resistance to various biological and abiotic stresses. In this study, 12 members of SRO gene family were identified from the genome of Gossypium hirsutum L.acc.TM-1 by bioinformatics. Multiple sequence alignment and phylogenetic tree analysis showed that all 12 Upland cotton SRO contained PARP and RST domains, which were grouped into three groups, namely, Agnb and C subfamilies. Transcriptional data analysis showed that GhD12G1442 in TM-1 genome expressed GhD05G2064 in stem, GhD05G2064 in sepals and stamen, GhA05G3788 in stamen, GhA12G1318 in stamen and GhA12G2480 in stamen and fiber, GhA12G2480, GhA05G2257 and GhD05G2516 in fiber. GhD 12G 2054 has a wide range of tissue expression. The results of stress treatment showed that GhA08G1390 was significantly induced by cold treatment and salt treatment.
【作者單位】： 安陽工學(xué)院;中國農(nóng)業(yè)科學(xué)院棉花研究所;棉花生物學(xué)國家重點(diǎn)實(shí)驗(yàn)室;
【基金】：河南省科技廳基礎(chǔ)與前沿項(xiàng)目(112300410019) 棉花生物學(xué)國家重點(diǎn)實(shí)驗(yàn)室開放課題(CB2014A10)共同資助~~
【分類號(hào)】：Q943.2;S562
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本文編號(hào)：2012303