本文選題：刺五加 + MDD基因�。� 參考：《中草藥》2016年21期

【摘要】：目的克隆并分析刺五加甲羥戊酸焦磷酸脫羧酶(mevalonate diphosphate decarboxylase,MDD)基因的啟動(dòng)子序列。方法根據(jù)刺五加MDD基因的c DNA序列,采用PCR擴(kuò)增和熱不對稱交錯(cuò)PCR(TAIL-PCR)技術(shù),克隆MDD基因5’端的DNA序列及啟動(dòng)子序列。利用Plant CARE等軟件對其進(jìn)行生物信息學(xué)分析。結(jié)果克隆得到長1 423 bp的刺五加MDD基因啟動(dòng)子序列及長1 024 bp的5’端DNA序列。該啟動(dòng)子序列含有49個(gè)TATA-box、25個(gè)CAAT-box。還含有脫落酸響應(yīng)元件、茉莉酸甲酯響應(yīng)元件、胚乳表達(dá)必須順式作用元件、干旱脅迫響應(yīng)元件、光響應(yīng)元件等多種順式作用元件,以及2個(gè)MYBHv1與2個(gè)MBY結(jié)合位點(diǎn)。結(jié)論首次克隆并分析了刺五加MDD基因的啟動(dòng)子序列,為該基因的表達(dá)調(diào)控奠定了基礎(chǔ)。
[Abstract]:Objective to clone and analyze the promoter sequence of mevalonate diphosphate decarboxylase (MDD) gene from Acanthopanax senticosus (Acanthopanax senticosus). Methods according to the cDNA sequence of MDD gene of Acanthopanax senticosus, the 5 '-terminal DNA sequence and promoter sequence of MDD gene were cloned by PCR amplification and thermal asymmetric interleaving PCR. The bioinformatics analysis was carried out by means of Plant care and other software. Results the promoter sequence of MDD gene of Acanthopanax senticosus and the 5 'terminal DNA sequence of 1 024 BP were cloned. The promoter contains 49 TATA-box and 25 CAAT-box. It also contains abscisic acid response element, methyl jasmonate response element, endosperm expression must be cis-acting element, drought stress response element, photoresponse element, and two MYBHv1 binding sites to two MBY. Conclusion the promoter sequence of MDD gene of Acanthopanax senticosus was cloned and analyzed for the first time.
【作者單位】： 華北理工大學(xué)生命科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(31570683) 河北省教育廳資助科研項(xiàng)目(QN2014102) 華北理工大學(xué)培育基金(SP201508);華北理工大學(xué)大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃(X2015170)
【分類號】：S567.19
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本文編號：2012256