本文選題：大環(huán)內(nèi)酯類化合物 + 聚酮合酶��； 參考：《天然產(chǎn)物研究與開(kāi)發(fā)》2017年11期

【摘要】：應(yīng)用Ⅰ型聚酮合酶(PKS-I)基因篩選體系從32株海洋放線菌中尋找大環(huán)內(nèi)酯類化合物的潛在產(chǎn)生菌,通過(guò)DNA序列相似性比對(duì)從5株陽(yáng)性菌株中篩選獲得了游動(dòng)放線菌Actinoplanes sp.FIM060065。采用HPLC制備色譜從該菌株發(fā)酵液中分離純化得到一個(gè)大環(huán)內(nèi)酯類化合物FW060065,紫外、質(zhì)譜以及核磁共振波譜分析表明它與臺(tái)勾霉素B同質(zhì),藥敏實(shí)驗(yàn)表明其對(duì)艱難梭菌、消化鏈球菌、雙歧桿菌等革蘭氏陽(yáng)性厭氧菌顯示出較強(qiáng)的抗菌能力。本研究通過(guò)基因篩選獲得了臺(tái)勾霉素的產(chǎn)生菌及其相應(yīng)的代謝產(chǎn)物,為Ⅰ型聚酮合酶基因與大環(huán)內(nèi)酯類化合物之間的聯(lián)系提供了證據(jù)。
[Abstract]:Using the type I polyketone synthase (PKS-I) gene screening system, we searched for potential macrolides producing bacteria from 32 strains of marine actinomycetes. Actinoplanes sp. FIM060065 was screened from 5 positive strains by DNA sequence similarity comparison. A macrolide compound FW060065 was isolated and purified by HPLC from the fermentation broth of the strain. UV, MS and NMR spectra showed that it was the same as tegoxymycin B, and the drug sensitivity test showed that it was resistant to Clostridium diffracta. Streptococcus peptidis, bifidobacterium and other Gram-positive anaerobic bacteria showed strong antibacterial ability. In this study, the production of tetracycline and its related metabolites were obtained by gene screening, which provided evidence for the relationship between the gene of type I polyketone synthase and macrolides.
【作者單位】： 福建省微生物研究所福建省新藥(微生物)篩選重點(diǎn)實(shí)驗(yàn)室;泉州醫(yī)學(xué)高等�？茖W(xué)校藥學(xué)系;
【基金】：福建省自然科學(xué)基金(2015J01291) 福建省海洋高新項(xiàng)目(2016-25) 福建省微生物分析檢測(cè)公共服務(wù)平臺(tái)建設(shè)(2013Y2003) 福建省化藥平臺(tái)項(xiàng)目(2014Y2001)
【分類號(hào)】：R915

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