本文選題：寧夏回漢兒童 + HLA基因多態(tài)性��； 參考：《寧夏醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的 探索寧夏地區(qū)回漢兒童的HLA-B、DRB1等位基因多態(tài)性,通過(guò)計(jì)算、分析寧夏地區(qū)回漢兒童HLA-B、DRB1等位基因的分布特點(diǎn),并與中醫(yī)體質(zhì)進(jìn)行統(tǒng)計(jì)分析,以探討HLA基因多態(tài)性與中醫(yī)體質(zhì)之間的關(guān)聯(lián),同期與其他人群的相關(guān)等位基因做比較,期待為本地區(qū)兒童過(guò)敏性紫癜及紫癜腎的發(fā)病及轉(zhuǎn)歸預(yù)測(cè)提供客觀的實(shí)驗(yàn)室依據(jù)。方法 采集寧夏地區(qū)76名回漢兒童的血樣,通過(guò)PCR-序列特異性引物(PCR-SSP)方法,對(duì)HLA-B、DRB1基因分型進(jìn)行檢測(cè),并對(duì)分型結(jié)果進(jìn)行統(tǒng)計(jì)分析,同期與其它人群數(shù)據(jù)做比較。與此同時(shí),我們對(duì)研究對(duì)象進(jìn)行中醫(yī)體質(zhì)的劃分,并且將HLA數(shù)據(jù)與中醫(yī)體質(zhì)進(jìn)行統(tǒng)計(jì)分析。結(jié)果 1.寧夏漢族HLA-B位點(diǎn)中共檢測(cè)到等位基因19種,基因頻率由高到低分別為B*15(16.3%)、B*35、40(13.0%)、B*13、46(9.8%)、B*44、51(5.4%)、B*50(4.3%)、B*07、37、48、52(3.3%)、B*08、55(2.2%)、HLA-B*18、38、49、54、57(1.1%)。HLA-DRB1位點(diǎn)中共檢測(cè)到等位基因13種。基因頻率由高到低分別為DRB1*12(19.6%)、DRB1*09(16.3%)、DRB1*07(12.0%)、DRB1*04、14(9.8%)、DRB1*15(8.7%)、DRB1*08(7.6%)、DRB1*13(4.3%)、DRB1*11、16(3.3%)、DRB1*03、10(2.2%)、DRB1*01(1.1%)。2.寧夏回族HLA-B位點(diǎn)中共檢測(cè)到等位基因19種,基因頻率由高到低分別為B*40、46(18.3%)、B*15(11.7%)、B*13(8.3%)、B*07、35、44、48、51(5.0%)、B*58(3.3%)、B*08、37、38、39、45、49、52、54、55(1.7%)。HLA-DRB1位點(diǎn)中共檢測(cè)到等位基因12種,基因頻率由高到低分別為DRB1*12(16.7%)、DRB1*15(15.0%)、DRB1*04、14(13.3%)、DRB1*11(11.7%)、DRB1*07、09、13(6.7%)、DRB1*03、08(3.3%)、DRB1*01、16(1.7%)。3.在寧夏漢族、回族人群中,HLA-B、HLA-DRB1等位基因頻率分布基本相似,差異無(wú)統(tǒng)計(jì)學(xué)意義。4.寧夏漢族人群的HLA-B、DRB1等位基因與山西漢族、山東漢族人群的分布情況基本相似,但是又具有自身的分布特點(diǎn)。結(jié)論 1.寧夏漢族與回族人群HLA-B、HLA-DRB1等位基因分布基本相似,差異無(wú)統(tǒng)計(jì)學(xué)意義;2.寧夏漢族人群HLA-B、HLA-DRB1等位基因與山西漢族、山東漢族基因頻率的分布大致相同,但又有其自身的分布特點(diǎn);3.可以推測(cè)HLA-DRB1*01/11為寧夏漢族HSP/HSPN患兒的易感基因,HLA-DRB1*07為抵抗基因;HLA-B*35、HLA-DRB1*01為寧夏回族HSP/HSPN患兒的易感基因,HLA-DRB1*07為抵抗基因;4.HLA具有明顯的種族和地域性差異,為群體遺傳學(xué)和HLA疾病相關(guān)性研究提供了重要的參考資料。
[Abstract]:Objective to explore the polymorphism of HLA-BmDRB1 alleles in Hui Han children in Ningxia, and to analyze the distribution characteristics of HLA-BnDRB1 alleles in Hui Han children in Ningxia, and to make statistical analysis with TCM constitution. In order to explore the association between HLA gene polymorphism and TCM physique, and to compare with other alleles in the same period, we hope to provide an objective laboratory basis for predicting the pathogenesis and prognosis of Henoch-Schonlein purpura and Henoch-Schonlein purpura kidney in children in this area. Methods Blood samples were collected from 76 Hui Han children in Ningxia area. The genotyping of HLA-BmDRB1 gene was detected by PCR-SSPP with PCR- sequence specific primers, and the typing results were statistically analyzed, and compared with other population data at the same time. At the same time, we classify the subjects of TCM constitution, and make statistical analysis of HLA data and TCM constitution. Result 1. A total of 19 alleles were detected at HLA-B locus in Han nationality in Ningxia. The frequencies of the alleles from high to low were BH151616.33B, B3554040 and 13.013.00.There were 13 alleles detected at B0737777777777772M3.3B and B08552.2in B08552.2and B08552.2B08552.2at B08552.2B, respectively. The gene frequencies from high to low were DRB1121219.6C and DRB10916.330.There was DRB10712.00.DRB1014149.8. DRB1158.7KT DRB1087.6D1RB1134.33DRB111111603102.2D0101K and DRB1011a / DRB1134.34.3t. 瀹佸鍥炴棌HLA-B浣嶇偣涓叡媯，

本文編號(hào)：2009847