本文選題：MTHFR酶 + 多態(tài)性　； 參考：《青海大學》2017年碩士論文

【摘要】：目的初步了解青海地區(qū)藏族原發(fā)性高血壓(Essential hypertension,EH)人群,血漿同型半胱氨酸(Homocysteine,Hcy)的濃度,明確Hcy的濃度差異是否與調(diào)節(jié)其代謝或運轉的酶基因多態(tài)性有關,并探討Hcy與亞甲基四氫葉酸還原酶(methylenetetrahydrofolate reductase,MTHFR)A1298C位點變異同青海地區(qū)藏族EH是否有因果關聯(lián),從而極大程度地為青海地區(qū)藏族EH的提前預警,針對性治療增加獨特的參考價值。方法收集2015.8-2016.12青海省人民醫(yī)院心血管內(nèi)科就診治療的藏族EH人群60例,男34例,女26例,平均年齡58.97±13.38歲,非EH組為2015.8-2016.12(相同時期)本院門診體檢的無高血壓青海藏族人群,年齡和性別與EH組分別相互對應,總計75例,男40例,女35例,平均年齡55.58±11.22歲。采集經(jīng)過EDTA抗凝的空腹靜脈血,盡量保證在一小時內(nèi)離心完畢,精密量取8μl血漿用Hcy測定儀速率法精密地定量測出Hcy水平;白細胞層嚴格遵從QIAGEN公司供給的Gentra Puregene Handbook操作獲得純化DNA,使用聚合酶鏈限制性內(nèi)切酶片段長度多態(tài)性(PCR-RFLP)進行目的片段擴增,檢測MTHFRA1298C的rs1801131位點變異情況,比較MTHFRA1298C基因型與等位基因在青海藏族EH組與非EH組的分布情況,及其相對應的Hcy水平特征,分析它們同EH是否存在因果關聯(lián)。結果(1)青海藏族高血壓組血漿Hcy水平為19.02±8.31μmol/L,高于對照組15.65±5.76μmol/l,且差異具有顯著性(P0.05)。青海藏族MTHFRA1298C各基因型AA的Hcy水平為17.42±6.33μmol/l、AC為19.47±8.24μmol/l、CC的16.45±7.30μmol/l,三者的濃度用One-Way ANOVA analysis進行對比,三者相互間差異都無顯著性(P=0.233)。通過多因子的二元Logistic回歸在控制血脂、體重指數(shù)混雜因子后,并未發(fā)現(xiàn)Hcy能作為EH的孤立風險因子單獨使其風險升高(OR=1.567,95%CI:0.513-4.788,P0.05)。(2)EH組MTHFRA1298C基因AA、AC和CC頻率分別為56.67%、38.33%、5.00%,A、C頻率分別為75.83%、24.17%;對照組AA、AC和CC頻率分別為61.33%、30.67%、8.00%,A、C分別為76.67%、23.33%,MTHFRA1298C的rs1801131位點的分布通過卡方檢驗,得出在青海地區(qū)藏族人群的EH組與對照組均無顯著差別(P值均大于0.05)。高血壓組MTHFRA1298C基因AA、AC+CC頻率分別56.67%、43.33%,對照組AA、AC+CC頻率分別61.33%、38.67%,兩組AA、AC+CC分布無顯著差別(P=0.583)。結論(1)EH組的血漿Hcy濃度顯著高于非EH組,然而,Hcy并不是EH的風險因子。(2)青海藏族MTHFRA1298C位點變異并不影響血漿Hcy濃度發(fā)生變化。(3)MTHFRA1298C位點變異同青海藏族EH人群并沒有顯著的因果關聯(lián)。
[Abstract]:Objective to investigate the plasma homocysteine (Hcy) concentration in the Tibetan essential hypertension (EH) population in Qinghai, and to determine whether the difference in Hcy concentration is related to the polymorphism of the enzyme gene that regulates its metabolism or operation. The relationship between the mutation of Hcy and methylenetetrahydrofolate reductase (MTHFRN A1298C) and Tibetan EH in Qinghai area was also discussed. The results indicated that Hcy and methylenetetrahydrofolate reductase (MTHFRN) A1298C mutation were causally related to the Tibetan EH in Qinghai area, thus providing a special reference value for Tibetan EH early warning and targeted therapy in Qinghai area. Methods A total of 60 Tibetan EH patients, 34 males and 26 females, with an average age of 58.97 鹵13.38 years, were collected from the Department of Cardiovascular Medicine of Qinghai Provincial people's Hospital from May to December, 2015.The non-EH group was an outpatient check-up group of non-EH Tibetans with no hypertension in Qinghai province during the same period from May 8 to June 12, 2015.The average age was 58.97 鹵13.38 years. Age and sex corresponded to each other in EH group (75 cases, male 40, female 35, mean age 55.58 鹵11.22 years old). Fasting venous blood after EDTA anticoagulant was collected to ensure that 8 渭 l plasma was accurately measured by Hcy rate method in an hour after centrifugation. The leukocyte layer was obtained by strictly following the Gentra Puregene Handbook operation provided by QIAGEN. Polymerase chain restriction fragment length polymorphism (PCR-RFLP) was used to amplify the target fragment, and the variation of rs1801131 loci in MTHFRA1298C was detected. The distribution of MTHFRA1298C genotype and allele in Qinghai Tibetan EH group and non-EH group were compared, and the corresponding Hcy level characteristics were compared, and the causal relationship between MTHFRA1298C genotype and EH was analyzed. Results (1) the plasma Hcy level in Qinghai Tibetan hypertension group was 19.02 鹵8.31 渭 mol / L, which was higher than that in the control group (15.65 鹵5.76 渭 mol / L), and the difference was significant (P 0.05). The homocysteine level of AA in each genotype of Qinghai Tibetan MTHFRA1298C was 17.42 鹵6.33 渭 mol / L and 19.47 鹵8.24 渭 mol / L, 16.45 鹵7.30 渭 mol / L, respectively. The concentrations of these three genotypes were compared with One-Way ANOVA analysis. There was no significant difference among the three genotypes (P0.233). After controlling for blood lipids and body mass index (BMI), multivariate logistic regression was used to analyze the relationship between blood lipids and body mass index. It was not found that Hcy can raise the risk of EH alone as an isolated risk factor for EH, and that the frequencies of AAC and CC of MTHFRA1298C gene in the EH group were 56.6767 ~ 38.335.005.005.005.005.005.00, respectively. The frequencies of AAC and CC in the control group were 61.3330.670.670.67and 8.00A respectively. The distribution of the rs1801131 site of MTHFRA1298C was 76.6767 ~ 23.333.339C, respectively. The results showed that there was no significant difference between the EH group and the control group in Qinghai Tibetan population (P > 0.05). The frequency of AAAC CC of MTHFRA1298C gene in hypertension group was 56.67 and that of control group was 61.338.67. there was no significant difference in the distribution of AAAC CC between the two groups. Conclusion the plasma Hcy concentration in the EH group is significantly higher than that in the non-EH group. However, Hcy is not a risk factor for EH.) the variation of MTHFRA1298C locus in Qinghai Tibetan does not affect the variation of plasma Hcy concentration. There is no significant causal correlation between the Hcy level variation and the Tibetan EH population in Qinghai.
【學位授予單位】：青海大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R544.11

【相似文獻】

相關期刊論文 前10條

1 寧靜;;糖尿病視網(wǎng)膜病變患者血漿HCY與血小板活化的關系[J];中國誤診學雜志;2007年27期

2 姬愛冬;柯冬云;鄒緘;;氣功對絕經(jīng)后血管性癡呆患者Hcy、Aβ干預作用的臨床觀察[J];長春中醫(yī)藥大學學報;2010年02期

3 高娟梅;岳巧蓮;張仲梅;劉克宇;;糖尿病微血管并發(fā)癥患者HCY臨床意義探究[J];大家健康(學術版);2014年12期

4 安翼;董志軍;李文麗;李明玉;;Hcy和血液流變學聯(lián)合檢測對老年急性腦血管病診斷的價值[J];中國老年學雜志;2012年20期

5 李靜;趙穎;;老年收縮期高血壓中醫(yī)證型與Hcy關系[J];中醫(yī)臨床研究;2011年04期

6 李偉峰;張玲端;李慶海;馬興榮;;缺血性中風急性期中醫(yī)證型特點及與血漿HCY的關系[J];中國中醫(yī)急癥;2009年08期

7 尹寶;李彥斌;韓立民;;高血壓病中醫(yī)辨證分型與血漿同型半胱氨酸(HCY)的相關性研究[J];贛南醫(yī)學院學報;2014年01期

8 陳燕銘;張曉菲;穆攀偉;王曼曼;林妙霞;曾龍驛;;Hcy在2型糖尿病合并脂肪肝及高血壓患者中的變化[J];中國衛(wèi)生檢驗雜志;2011年06期

9 陳暉;郭媛;;2型糖尿病視網(wǎng)膜病變患者檢測HCY和血流變學指標的意義[J];海南醫(yī)學院學報;2012年03期

10 吳志美;許亮亮;王偉鵬;;Hcy、VWF在糖尿病視網(wǎng)膜病變患者中的水平及關系[J];牡丹江醫(yī)學院學報;2010年05期

相關會議論文 前5條

1 趙偉;唐愛華;李吉武;陳松林;陳文輝;唐春麗;劉春紅;翟琳娜;王妍;陳莉;;2型糖尿病Hcy與濁毒的相關性及濁毒清對Hcy的影響[A];第十五次全國中醫(yī)糖尿病大會論文集[C];2014年

2 遲偉群;姚玉虹;于曉斐;趙明;;HCY與hsCRP聯(lián)合檢測在評價冠狀動脈粥樣硬化風險中的作用[A];2009年全國微循環(huán)與血液流變學基礎研究及臨床應用學術研討會專題報告及論文集[C];2009年

3 陳峻;胡必成;王唯;;2型糖尿病患者血清胱抑素C和Hcy檢測的臨床價值[A];中華醫(yī)學會第九次全國檢驗醫(yī)學學術會議暨中國醫(yī)院協(xié)會臨床檢驗管理專業(yè)委員會第六屆全國臨床檢驗實驗室管理學術會議論文匯編[C];2011年

4 周國忠;斯徐偉;葉飛;倪鐵鈞;;2型糖尿病伴眼底病變者HCY等水平變化的相關研究[A];2007年浙江省醫(yī)學檢驗學學術年會論文匯編[C];2007年

5 賈敬年;胡小珊;;血清同型半胱氨酸(Hcy)與高血壓及腦梗死的關系研究[A];2006年浙江省檢驗醫(yī)學學術年會論文匯編[C];2006年

相關重要報紙文章 前2條

1 張驍；束梅英;HCY是冠心病發(fā)病的獨立危險因素[N];中國醫(yī)藥報;2004年

2 葛宗漁;Hcy是高血壓患者中風的大幫兇[N];光明日報;2008年

相關博士學位論文 前1條

1 李艾帆;Hcy及其代謝關鍵酶基因多態(tài)性與河南漢族腦梗死的相關性研究[D];鄭州大學;2014年

相關碩士學位論文 前10條

1 陳單單;青海藏族高血壓患者Hcy及其代謝酶基因多態(tài)性研究[D];青海大學;2017年

2 劉玲玲;抵抗素、HCY等與冠心病的相關性研究[D];山東大學;2005年

3 王剛華;血漿HCY及其代謝酶基因多態(tài)性與神經(jīng)管畸形的關系[D];山西醫(yī)科大學;2006年

4 齊敬東;補陽還五湯對中風病氣虛血瘀證HCY影響的研究[D];山東中醫(yī)藥大學;2011年

5 陳先卓;柚皮苷對Hcy所致人臍靜脈內(nèi)皮細胞凋亡的抑制作用研究[D];川北醫(yī)學院;2010年

6 蔣群龍;HCY、MMP-9/TIMP-1失衡與頸動脈粥樣硬化斑塊穩(wěn)定性的關系[D];泰山醫(yī)學院;2013年

7 常佳;血漿中HCY、葉酸、維生素B12水平與腦梗塞及血管性癡呆的相關性研究[D];吉林大學;2014年

8 孫巧思;芪桂顆粒對甲狀腺功能減退癥大鼠HCY，，hs-CRP的影響[D];黑龍江省中醫(yī)藥科學院;2014年

9 沈媛;腦梗死患者Hcy、MTHFR基因多態(tài)性與頸動脈硬化[D];首都醫(yī)科大學;2007年

10 鄭世雯;不同濃度HCY對培養(yǎng)的人臍靜脈內(nèi)皮細胞eNOS及ET-1轉錄水平的影響[D];中國醫(yī)科大學;2005年

本文編號：2009024