發(fā)布時(shí)間：2018-06-12 06:36

  本文選題：白木香 + 人工誘導(dǎo)�。� 參考：《時(shí)珍國醫(yī)國藥》2017年05期

【摘要】：目的研究不同人工誘導(dǎo)方法對(duì)白木香結(jié)香過程中萜類相關(guān)基因表達(dá)量的影響。方法 "小孔滴注法"進(jìn)行甲酸、甲酸結(jié)合鐮刀菌人工誘導(dǎo)試驗(yàn),手工割取誘導(dǎo)前、后12個(gè)月內(nèi)的白木香結(jié)香部位,實(shí)時(shí)熒光定量PCR法(qRT-PCR)測(cè)定As-HMGR、As-DXS1、As-DXS2基因表達(dá)水平。結(jié)果 As-HMGR、As-DXS1、As-DXS2回歸方程分別為Y=-3.580X+40.11、Y=-3.424X+38.38、Y=-3.728X+41.84,相關(guān)系數(shù)均大于0.997,線性關(guān)系均良好。甲酸、甲酸結(jié)合鐮刀菌誘導(dǎo)過程中,As-DXS1與As-DXS2的表達(dá)模式相近,前8個(gè)月表達(dá)水平較低,隨后顯著升高。甲酸誘導(dǎo)時(shí),As-HMGR基因從2個(gè)月開始表達(dá),呈現(xiàn)先升高后下降再升高的趨勢(shì);甲酸結(jié)合鐮刀菌誘導(dǎo)時(shí),初期相對(duì)表達(dá)量緩慢升高,6個(gè)月時(shí)達(dá)到峰值,隨后急劇下降。人工誘導(dǎo)12個(gè)月As-HMGR、As-DXS1的相對(duì)表達(dá)量,甲酸誘導(dǎo)高于甲酸結(jié)合鐮刀菌誘導(dǎo)(P0.05)。結(jié)論甲酸、甲酸結(jié)合鐮刀菌誘導(dǎo)白木香結(jié)香過程中As-HMGR基因在傷害早期響應(yīng)傷害脅迫,As-DXS1、As-DXS2基因在后期響應(yīng),為進(jìn)一步研究白木香萜類次生代謝產(chǎn)物表達(dá)與調(diào)控,改善人工沉香品質(zhì)奠定基礎(chǔ)。
[Abstract]:Objective to study the effect of different artificial induction methods on the expression of terpene related genes in the process of aroma formation. Methods Formic acid, formic acid combined with Fusarium mongolicum were used for formic acid, formic acid and Fusarium oxysporum to detect the expression level of As-DXS1As-DXS2 gene in As-HMGRN As-DXS1As-DXS2 gene by real-time fluorescence quantitative PCR within 12 months before and 12 months after induction. Results the regression equations of As-HMGRN As-DXS1 and As-DXS2 were Yan-3.580X 40.11X 40.11X 38.324X 38.324X 38.328X 41.84, and the correlation coefficients were all greater than 0.997.The linear relationship was good. The expression patterns of As-DXS1 and As-DXS2 were similar to those of As-DXS2 during the induction of formic acid and formic acid-bound Fusarium. The expression level of As-DXS1 was lower in the first eight months and then increased significantly. The expression of As-HMGR gene began from 2 months after formic acid induction, which increased first and then decreased and then increased. When formic acid was induced by Fusarium oxysporum, the relative expression level increased slowly, reached its peak at 6 months, and then decreased sharply. The relative expression of As-HMGRN As-DXS1 in artificial 12 months was higher than that induced by Fusarium formate. Conclusion in the process of formic acid, formic acid combined with Fusarium, As-HMGR gene responds to injury stress in the early stage of injury. In order to further study the expression and regulation of terpenoid secondary metabolites, As-DXS1 and As-DXS2 genes are in late response to injury stress. Improve the quality of artificial incense to lay the foundation.
【作者單位】： 廣東藥科大學(xué)藥學(xué)院;仙樂健康科技股份有限公司;廣東省微生物研究所省部共建華南應(yīng)用微生物國家重點(diǎn)實(shí)驗(yàn)室廣東省菌種保藏與應(yīng)用重點(diǎn)實(shí)驗(yàn)室廣東省微生物應(yīng)用新技術(shù)公共實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金(No.81102418) 廣東省基礎(chǔ)與應(yīng)用基礎(chǔ)研究專項(xiàng)資金(No.2014A030313583) 廣東省中醫(yī)藥局科研課題(No.20161151) 廣東省科學(xué)院野外科學(xué)實(shí)驗(yàn)站基金項(xiàng)目(No.Sytz201504;No.Sytz201511)
【分類號(hào)】：S567.19

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