本文選題：急性濕疹 + 馬齒莧鮮榨液��； 參考：《成都中醫(yī)藥大學(xué)》2016年碩士論文

【摘要】：目的：觀察兩種不同馬齒莧提取物有效成分對急性濕疹大鼠背部皮膚組織HRH1和Filaggrin基因表達(dá)的影響,探討不同馬齒莧提取物治療急性濕疹的可能機(jī)制。方法：SD大鼠50只,♂早各半,隨機(jī)分為5組,每組10只,即：正常組、模型組、陽性藥物對照組、馬齒莧醇提取物組、馬齒莧鮮榨液組。除正常組外,用電剃須刀剃去其余四組大鼠腹部毛發(fā),面積約1 cm×2 cm,并在大鼠剃毛部位涂抹5%的2,4-二硝基氯苯(DNCB) 30μl于皮膚首次致敏;在致敏后第三天,再用電剃須刀剃去左、右對稱背部約為2cm×2cm面積的剃毛區(qū)域,并在右背部剃毛區(qū)涂DNCB進(jìn)行二次抗原攻擊,濃度為0.2%,用量為50μ1,左背部對稱的剃毛區(qū)不用藥物,作為對照部位。以后按上述方法連續(xù)激發(fā)三次,每三天一次,用藥后第九天觀察大鼠右背部皮膚明顯出現(xiàn)紅斑,水腫,丘疹,糜爛或滲出,提示造模成功。于激發(fā)后第十天開始給藥,每天兩次,每次1ml,連續(xù)九天,最后在首次致敏后的第二十一天斷頸處死大鼠,剪取造模區(qū)皮膚,分別予RT-PCR檢測各實(shí)驗(yàn)組大鼠皮膚Filaggrin基因表達(dá)和免疫組化檢測HRH1平均光密度。結(jié)果：1、與正常組相比,模型組大鼠皮膚組織HRH1蛋白含量明顯升高,具有非常顯著的統(tǒng)計(jì)學(xué)意義(P0.01),與模型組相比,馬齒莧鮮榨液組大鼠皮膚組織HRH1蛋白含量明顯降低,具有非常顯著的統(tǒng)計(jì)學(xué)意義(P0.01),陽性對照組、馬齒莧醇提物組大鼠皮膚組織HRH1蛋白含量降低,具有統(tǒng)計(jì)學(xué)意義(P0.05)。2、與正常組相比,模型組Filaggrin基因的2~(-△△CT)值極顯著小(P0.01),與模型組比較,馬齒莧鮮榨液組和陽性對照組大鼠皮膚組織細(xì)胞中Filaggrin基因的2~(-△△CT)值極顯著增大(P0.01),馬齒莧醇提取物組大鼠皮膚組織細(xì)胞中Filaggrin基因的2~(-△△CT)值極顯著增大(P0.05)。結(jié)論：兩種馬齒莧提取物都可以使血管收縮,抑制毛細(xì)血管通透性,抑制炎性腫脹,從而減輕濕疹局部的瘙癢、水腫癥狀。尤其是馬齒莧鮮榨液效果更好。其機(jī)制可能是兩種馬齒莧提取物能夠顯著的降低大鼠皮膚組織中瘙癢因子HRH1的水平,明顯控制大鼠搔抓反應(yīng)和改善了皮損情況,同時兩種馬齒莧提取物上調(diào)濕疹動物皮膚屏障系統(tǒng)中Filaggrin基因的表達(dá),加速Filaggrin水解成各種天然保濕因子,有效改善和修復(fù)大鼠皮膚屏障功能,增強(qiáng)了機(jī)體的免疫功能,從而起到了治療急性濕疹的作用。
[Abstract]:Objective: to observe the effects of two effective components of purslane extract on the expression of HRH1 and Filaggrin gene in the dorsal skin of rats with acute eczema, and to explore the possible mechanism of different extracts of Portulaca oleracea for the treatment of acute eczema. Methods Fifty Sprague-Dawley rats were randomly divided into 5 groups: normal group, model group, positive drug control group, ethanol extract group, fresh extract group of Portulaca oleracea and fresh extract of Portulaca oleracea. In addition to the normal group, the abdominal hair of the other four groups was shaved with electric razor, the area of which was about 1 cm 脳 2 cm, and the skin was sensitized with 5% of DNCB30 渭 l on the shaving area of the rats, and the left was shaved with the razor on the third day after sensitization. The right symmetrical back is about the shaving area of 2cm 脳 2cm, and the second antigenic attack is carried out in the shaving area of the right back. The concentration is 0.2and the dosage is 50 渭 1. The symmetrical shaving area of the left back is not used as the control site. The results showed that the skin of the right back of the rats appeared erythema, edema, papules, erosion or exudation on the ninth day after treatment, indicating that the model was successful. On the tenth day after stimulation, the rats were given drugs twice a day, 1ml / time for nine days. Finally, the rats were killed at the 21 day after the first sensitization, and the skin of the model area was cut off. The expression of Filaggrin gene was detected by RT-PCR and the mean optical density of HRH1 was detected by immunohistochemistry. Results compared with the normal group, the content of HRH1 protein in the skin tissue of the model group was significantly higher than that of the normal group, and the content of HRH1 protein in the skin tissue of the model group was significantly lower than that of the model group, and the content of HRH1 protein in the skin tissue of the model group was significantly lower than that of the model group. The content of HRH1 protein in the skin of rats in the positive control group and the ethanol extract group of Portulaca oleracea was significantly lower than that in the normal group, and the content of HRH1 protein in the skin tissue of the rats was significantly lower than that in the normal group. The value of Filaggrin gene in the model group was significantly lower than that in the model group (P 0.01), and was significantly lower than that in the model group. The value of Filaggrin gene in skin tissue cells of fresh extract group and positive control group was significantly increased (P 0.01), and that of Filaggrin gene in skin tissue cells of purslane alcohol extract group was significantly increased (P 0.05). Conclusion: both extracts of Portulaca oleracea can induce vasoconstriction, inhibit capillary permeability, inhibit inflammatory swelling, and relieve local itching and edema in eczema. Especially the fresh extract of Portulaca oleracea was better. The mechanism may be that the two extracts of Portulaca oleracea can significantly reduce the level of pruritus factor HRH1 in the skin tissue of rats, control the scratching reaction of rats and improve the condition of skin lesions. At the same time, two extracts of Portulaca oleracea upregulated the expression of Filaggrin gene in the skin barrier system of eczema animals, accelerated the hydrolysis of Filaggrin into various natural moisturizing factors, effectively improved and repaired the skin barrier function of rats, and enhanced the immune function of the body. Thus played a role in the treatment of acute eczema.
【學(xué)位授予單位】：成都中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R285.5

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