本文選題：小麥 + 抗病��； 參考：《作物學(xué)報》2016年06期

【摘要】：NBS(nucleotide binding site)類基因是植物界中最重要的一類抗病基因。用信息學(xué)方法從普通小麥(Triticum aestivum L.)全基因組中分離出2406條含有NBS結(jié)構(gòu)的完整蛋白序列,每條包含48~2272個氨基酸殘基。根據(jù)NBS結(jié)構(gòu)域兩端是否連接CC或LRR結(jié)構(gòu)域,將TaNBS分為N、CN、NL和CNL 4類。對TaNBS所在scaffold序列的SSR位點進(jìn)行診斷,從1203條scaffold序列上發(fā)現(xiàn)2177個SSR位點,以二堿基重復(fù)位點最多,占73.5%。針對小麥2AL染色體上的51個SSR位點開發(fā)標(biāo)記,缺體 四體和雙端體驗證結(jié)果表明,有39個標(biāo)記(76.5%)為2AL特異標(biāo)記,其中24個特異標(biāo)記在抗白粉病材料Khapli(2AL上攜帶Pm4a)和感病材料Chancellor間存在多態(tài)性。利用近等基因系Khapli/8*Cc篩選出3個可能與Pm4a連鎖的NBS-SSR標(biāo)記,分別是Sxaas_2AL22、Sxaas_2AL39和Sxaas_2AL46。本研究開發(fā)的與抗病序列緊密連鎖的特異SSR標(biāo)記可用于2AL染色體上抗病新基因的檢測以及已有抗病基因的候選序列篩選。
[Abstract]:Nucleotide binding site- like genes are the most important resistance genes in plant. Study on Triticum aestivum L. from Triticum aestivum L. by means of informatics 2406 complete protein sequences containing NBS structure were isolated from the whole genome, each containing 48 ~ 2272 amino acid residues. According to whether the two ends of the NBS domain are connected with CC or LRR domain, TaNBS is classified into NNCNNNL and CNL4. The scaffold loci of TaNBS were diagnosed and 2177 SSR loci were found from 1203 scaffold sequences. The most of them were two base repeats (73.5%). According to 51 SSR loci on wheat 2AL chromosome, the results of tetralogy and biterminal experience showed that 39 markers (76. 5) were 2AL specific markers. Among them, 24 specific markers carried Pm4a on the powdery mildew resistant material Khaplizao 2AL) and there was polymorphism between the susceptible material Chancellor and the anti-powdery mildew resistant material Khapliao 2AL. Three NBS-SSR markers, Sxaas2AL22, Sxaas2AL39 and Sxaas2AL46, which may be linked to Pm4a, were screened by using the near isogenic line Khapli-8Cc. The specific SSR markers closely linked to disease-resistant sequences can be used to detect new resistant genes on chromosome 2AL and to screen candidate sequences of disease-resistant genes.
【作者單位】： 山西大學(xué)生命科學(xué)學(xué)院;山西省農(nóng)業(yè)科學(xué)院作物科學(xué)研究所/作物遺傳與分子改良山西省重點實驗室;山西省農(nóng)業(yè)科學(xué)院旱地農(nóng)業(yè)研究中心;北京市農(nóng)林科學(xué)院雜交小麥工程技術(shù)研究中心;山西省農(nóng)業(yè)科學(xué)院小麥研究所;
【基金】：國家自然科學(xué)基金項目(31171839,31401385) 山西省青年基金項目(2015021145) 山西省農(nóng)業(yè)攻關(guān)項目(20150311001-1,20150311001-5) 山西省農(nóng)業(yè)科學(xué)院攻關(guān)項目(15YGG01) 北京市農(nóng)林科學(xué)院青年基金項目(QNJJ201428)資助~~
【分類號】：S512.1
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本文編號：1992566