本文選題：紫蘇 + 迷迭香酸��； 參考：《分子植物育種》2016年02期

【摘要】：以前期獲得的紫蘇HPPR基因的c DNA序列為模板,通過設計特異性引物和染色體步移的方法分離出HPPR基因啟動子,全長2 216 bp,以此研究HPPR基因啟動子的結(jié)構(gòu)及功能特點。生物信息學分析表明,該序列中存在TATA-box和CAAT-box等典型的真核生物啟動子基本元件,以及對茉莉酸甲酯、生長素、水楊酸和脫落酸等植物激素應答相關(guān)的順式作用元件,另外也存在非生物脅迫順式作用元件。將HPPR啟動子部分缺失序列替代p BI121載體中的Ca MV35S啟動子,構(gòu)建了與GUS融合的啟動子元件缺失表達載體。本試驗的研究為該啟動子在今后紫蘇轉(zhuǎn)基因定向改良提供了有用的候選資源。
[Abstract]:Using the cDNA sequence of HPPR gene obtained in the early stage as template, the promoter of HPPR gene was isolated by designing specific primers and chromosome step method. The promoter was 2216 BP in length. The structure and functional characteristics of HPPR gene promoter were studied. Bioinformatics analysis showed that there were typical eukaryotic promoter elements such as TATA-box and CAAT-box, and cis-acting elements related to phytohormone responses such as methyl jasmonate, auxin, salicylic acid and abscisic acid. There are also abiotic stress cis-acting elements. The partial deletion sequence of HPPR promoter was replaced by the Ca MV35S promoter in pBI121 vector, and the expression vector with Gus fusion promoter element deletion was constructed. This study provides a useful candidate resource for the future directed modification of perilla.
【作者單位】： 天津科技大學食品工程與生物技術(shù)學院;
【基金】：國家863高技術(shù)研究發(fā)展計劃(2007AA100401)資助
【分類號】：S567.219

【相似文獻】

相關(guān)期刊論文 前10條

1 郝迪，

本文編號：1981789