本文選題：大白菜(Brassica + rapa　； 參考：《中國(guó)油料作物學(xué)報(bào)》2017年04期

【摘要】：為了解白菜品種抗性特征和抗病基因類型之間的關(guān)系,以我國(guó)油菜主產(chǎn)區(qū)存在的2、4和13號(hào)小種,以及來(lái)自湖北恩施、四川德陽(yáng)、河北唐山、河南南陽(yáng)4個(gè)地點(diǎn)的未知小種為病原,采用溫室人工接種方法,鑒定了28個(gè)大白菜品種對(duì)8個(gè)不同根腫菌菌株抗病性,同時(shí)利用已知的7個(gè)抗根腫病基因(Crr1、Crr2、Crr3、CRa、CRb、CRc、CRk)分子標(biāo)記檢測(cè)不同品種所含的抗病基因位點(diǎn)。研究結(jié)果表明:供試的28個(gè)大白菜品種,27個(gè)品種對(duì)不同菌株表現(xiàn)出一定的抗病性,品種根王F1對(duì)所有菌株表現(xiàn)感病。CR春福等10個(gè)品種對(duì)所有菌株表現(xiàn)抗性;CR-涼春等12個(gè)品種只對(duì)一個(gè)菌株感病,其中9個(gè)品種只對(duì)恩施菌株感病。分子標(biāo)記檢測(cè)結(jié)果顯示,感病品種根王F1中未檢測(cè)到抗病基因位點(diǎn),其余27個(gè)大白菜品種共檢測(cè)到Crr2、Crr3、CRa、CRb、CRk五個(gè)抗病位點(diǎn),CRk在抗病品種中普遍存在,但Crr3僅在CR春福中檢測(cè)到雜合抗病位點(diǎn)�？傮w而言,抗病位點(diǎn)較多的品種對(duì)不同地方菌株抗性強(qiáng),少數(shù)品種存在例外,包括含有多個(gè)位點(diǎn)仍表現(xiàn)感病(含有4個(gè)抗病位點(diǎn)的CR-黃心F1對(duì)4號(hào)、2號(hào)、13號(hào)生理小種表現(xiàn)感病)和僅含有1個(gè)位點(diǎn)但表現(xiàn)抗病(德高CR117和榮耀只檢測(cè)到CRk抗病位點(diǎn)但對(duì)所有菌株都表現(xiàn)出抗性)兩類情形。說(shuō)明大白菜對(duì)根腫病抗性及植物病原互作的復(fù)雜性,有待深入研究。
[Abstract]:In order to understand the relationship between resistance characteristics and resistance gene types of Chinese cabbage varieties, races 2Q4 and 13 existed in the main rapeseed producing areas of China, as well as from Enshi, Sichuan, Deyang, Hebei, Tangshan, Hebei, were studied. Unknown races from 4 locations in Nanyang, Henan Province were used to identify 28 varieties of Chinese cabbage resistant to 8 different rhizomegaly strains by means of artificial inoculation in greenhouse. At the same time, seven known gene markers for resistance to root-swelling were used to detect the resistance loci of different varieties. The results showed that 27 of 28 varieties of Chinese cabbage showed resistance to different strains. Root Wang F1 was susceptible to all strains. CR Chunfu and other 10 varieties showed resistance to all strains. 12 varieties, such as CR-Liangchun, were only susceptible to one strain, 9 of which were only susceptible to Enshi strain. The results of molecular marker analysis showed that no resistance gene loci were detected in susceptible cultivar GenwangF1, and the other 27 Chinese cabbage cultivars were found to have five resistance loci, CRK and CRK, which were commonly found in resistant varieties. However, Crr3 only detected heterozygous resistance sites in CR. Generally speaking, the varieties with more resistant sites had strong resistance to different local strains, with the exception of a few varieties. These include multiple loci that are still susceptible to disease (CR-Huangxin F1 with 4 disease-resistant loci to 4, 2, 13 physiological races) and only one locus to show disease resistance (Deco CR117 and Glory only detected CRk disease resistance). Loci but resistant to all strains). The complexity of the interaction between resistance to root-swelling and plant pathogens in Chinese cabbage needs to be further studied.
【作者單位】： 中國(guó)農(nóng)業(yè)科學(xué)院油料作物研究所/農(nóng)業(yè)部油料作物生物學(xué)與遺傳育種重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(31401720,31101124) 國(guó)家重點(diǎn)研發(fā)項(xiàng)目(2016YFD0100200) 國(guó)家油菜產(chǎn)業(yè)技術(shù)體系(CARS-13)
【分類號(hào)】：S436.341

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