發(fā)布時(shí)間：2018-06-03 23:43

  本文選題：CCCH鋅指蛋白 + 非生物脅迫�。� 參考：《分子植物育種》2016年09期

【摘要】：CCCH型鋅指蛋白是一類生物體中廣泛存在的轉(zhuǎn)錄因子,在多種植物生物學(xué)過程中起著關(guān)鍵的調(diào)控作用。在水稻全基因組中已發(fā)現(xiàn)67個(gè)CCCH基因,分為8個(gè)亞家族,而其中大部分基因的功能尚缺少研究。本研究通過熒光定量RT-PCR技術(shù)分析了水稻Os CCCH家族基因的組織表達(dá)模式以及鹽、PEG、低溫等非生物脅迫和脫落酸(ABA)對(duì)CCCH基因的誘導(dǎo)表達(dá)特征。結(jié)果表明,大部分Os CCCH基因在各組織中有不同的表達(dá)模式,約30%基因?yàn)榈拓S度表達(dá),50%以上基因表現(xiàn)為中豐度表達(dá),極少Os CCCH基因?yàn)楦哓S度表達(dá),包括Os C3H2、Os C3H35、Os C3H41、Os C3H43、Os C3H58和Os C3H67這6個(gè)組織優(yōu)勢(shì)表達(dá)基因。多數(shù)Os CCCH基因都不同程度地受到鹽、PEG、低溫及ABA的誘導(dǎo)或抑制,表明這些CCCH鋅指蛋白可能參與水稻的非生物脅迫響應(yīng);在四種脅迫下,有7個(gè)基因同時(shí)受到誘導(dǎo),其中Os C3H17基因上調(diào)倍數(shù)高達(dá)17倍以上;有10個(gè)基因同時(shí)被抑制,其中Os C3H16基因下調(diào)倍數(shù)高達(dá)31倍以上。
[Abstract]:Zinc finger protein (CCCH) is a widespread transcription factor in a variety of organisms and plays a key role in many plant biological processes. 67 CCCH genes have been found in the whole rice genome, which are divided into 8 subfamilies, but the function of most of these genes has not been studied. In this study, the tissue expression patterns of rice Os CCCH family genes and the induced expression characteristics of CCCH gene under abiotic stress, such as low temperature and abscisic acid (Aba), were analyzed by fluorescence quantitative RT-PCR. The results showed that most of the Os CCCH genes had different expression patterns in different tissues, about 30% of the genes expressed in low abundance and over 50% of the genes expressed in medium abundance, and very few of the Os CCCH genes were expressed in high abundance. There are six dominant expression genes including Os C3H35OC3H41OC3H41OOC3H43OOs C3H58 and Os C3H67. Most of Os CCCH genes were induced or inhibited in different degrees by salt peg, low temperature and ABA, indicating that these CCCH zinc finger proteins may be involved in abiotic stress response of rice, and 7 genes were induced simultaneously under four stresses. The up-regulated multiple of Os C3H17 gene was more than 17 times, and 10 genes were inhibited at the same time, in which Os C3H16 gene down-regulated by more than 31 times.
【作者單位】： 重慶市農(nóng)業(yè)科學(xué)院生物技術(shù)研究中心逆境農(nóng)業(yè)研究重慶市重點(diǎn)實(shí)驗(yàn)室;
【基金】：重慶市農(nóng)業(yè)科學(xué)院博士啟動(dòng)項(xiàng)目資助
【分類號(hào)】：Q943.2
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本文編號(hào)：1974778