本文選題：白木香 + sHSP基因��； 參考：《中草藥》2016年22期

【摘要】：目的從白木香Aquilaria sinensis中克隆sHSP1和sHSP2基因,并對它們的生物信息學及表達模式進行分析。方法根據(jù)本課題組白木香轉(zhuǎn)錄組數(shù)據(jù)庫進行分析獲得2條具有小分子熱激蛋白(sHSPs)保守結(jié)構(gòu)域的sHSPs基因序列,利用RT-PCR技術(shù)克隆sHSP1和sHSP2基因的全長c DNA序列,并對其進行生物信息學分析;利用實時熒光定量PCR檢測sHSP1和sHSP2基因在不同組織和高鹽及外源ABA、SA、MJ處理下不同時間的表達差異。結(jié)果克隆得到的白木香sHSP1和sHSP2基因開放閱讀框都為474 bp,編碼157個氨基酸。組織表達分析的結(jié)果顯示,sHSP1和sHSP2基因主要在根中表達,在莖和葉中的表達量較少;白木香愈傷組織在高鹽處理下,sHSP1和sHSP2基因分別在36 h和24 h達到最高表達水平,而愈傷組織在外源ABA、MJ處理下,sHSP1和sHSP2基因都在12 h達到最高表達水平,在SA處理下,sHSP1和sHSP2基因分別在12、24 h達到最高表達水平。結(jié)論獲得了sHSP1和sHSP2 2基因全長c DNA序列,且2個基因在不同組織根、莖、葉中的表達存在差異性,在受到高鹽和外源ABA、SA、MJ處理時,在不同時間的愈傷組織中的表達及積累情況也不同,該研究為后續(xù)深入研究白木香防御反應奠定了基礎(chǔ)。
[Abstract]:Objective to clone sHSP1 and sHSP2 genes from Aquilaria sinensis and analyze their bioinformatics and expression patterns. Methods two sHSPs gene sequences with small molecular heat shock protein (HSPs) conserved domain were obtained by analyzing the transcriptional database of the white wood incense. The full-length c DNA sequences of sHSP1 and sHSP2 genes were cloned by RT-PCR technique. The expression of sHSP1 and sHSP2 genes in different tissues, high salt and exogenous ABASAMJ were detected by real-time fluorescence quantitative PCR. Results the open reading frames of sHSP1 and sHSP2 genes were 474 BP, encoding 157 amino acids. The results of tissue expression analysis showed that HSP1 and sHSP2 genes were mainly expressed in roots, but less in stems and leaves, and the highest expression levels of HSP1 and sHSP2 genes were found in callus of Aucklanthus chinensis under high salt treatment at 36h and 24h, respectively. However, the highest expression level of HSP1 and sHSP2 genes in callus was reached at 12 h after exogenous ABAA-MJ treatment, and the highest expression level of HSP1 gene and sHSP2 gene were reached at 1224 h after SA treatment. Conclusion the full-length c DNA sequences of sHSP1 and sHSP2 2 genes were obtained, and there were differences in the expression of the two genes in root, stem and leaf of different tissues. The expression and accumulation of callus were different at different time, which laid a foundation for further study on the defense response of Amur.
【作者單位】： 北京中醫(yī)藥大學中藥學院;北京中醫(yī)藥大學中藥現(xiàn)代研究中心;
【基金】：北京市自然科學基金青年項目(7154216)
【分類號】：S567.19

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