本文選題：牛 + 早熟性　； 參考：《畜牧獸醫(yī)學報》2016年09期

【摘要】：旨在研究不同類型牛GPR54基因啟動子的變異對其表達水平的影響,以揭示牛GPR54基因多態(tài)性與牛早熟性之間的關聯(lián)性。提取荷斯坦牛、皖東黃牛及江淮水牛3種牛血液白細胞總RNA,通過PCR、測序、RT-PCR和雙熒光素酶報告基因的方法,檢測不同牛GPR54基因啟動子區(qū)的多態(tài)性,啟動子效率以及GPR54基因的表達水平,分析啟動子變異在牛品種、年齡和性別間對GPR54基因表達的效應。結果表明,GPR54基因啟動子存在多個SNP位點,3種牛的啟動子型存在明顯的差異,其中荷斯坦牛GPR54基因啟動子效率是江淮水牛的4.44倍,是皖東黃牛的1.99倍,而皖東黃牛啟動子效率是江淮水牛的2.24倍。RT-PCR顯示,青年牛GPR54基因表達水平高于成年牛;荷斯坦牛高于皖東黃牛和水牛,水牛的GPR54表達水平最低;母牛的GPR54基因表達水平極顯著高于公牛(P0.01)。綜上表明,不同牛的早熟性與GPR54啟動子存在密切的關聯(lián)性。
[Abstract]:The aim of this study was to study the effect of the promoter variation of bovine GPR54 gene on the expression level of bovine GPR54 gene in order to reveal the association between bovine GPR54 gene polymorphism and bovine precocious puberty. Three kinds of white blood cell total RNAs were extracted from Holstein cattle, Anhui Yellow cattle and Jianghuai buffalo. The polymorphisms of the promoter region of different bovine GPR54 gene were detected by PCR, sequencing RT-PCR and double luciferase reporter gene method. The efficiency of promoter and the expression level of GPR54 gene were analyzed, and the effect of promoter variation on GPR54 gene expression between cattle breeds, age and sex was analyzed. The results showed that there were significant differences among the three SNP loci in the promoter of GPR54 gene, and the promoter efficiency of GPR54 gene in Holstein cattle was 4.44 times of that in Jianghuai buffalo and 1.99 times of that in East Anhui cattle. The promoter efficiency of East Anhui cattle was 2.24 times higher than that of Jianghuai buffalo. RT-PCR showed that the GPR54 gene expression level of young cattle was higher than that of adult cattle, and that of Holstein cattle was higher than that of Anhui East Yellow cattle and water buffalo, and the GPR54 expression level of water buffalo was the lowest. The expression level of GPR54 gene in cows was significantly higher than that in bulls (P 0.01). In conclusion, the early maturation of different cattle was closely related to the GPR54 promoter.
【作者單位】： 安徽農(nóng)業(yè)大學動物科技學院;安徽畜禽遺傳資源保護與生物育種省級實驗室;
【基金】：國家自然科學基金項目(31272402)
【分類號】：S823.2

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