本文選題：白樺 + -羥基--甲基戊二酰輔酶A還原酶　； 參考：《中草藥》2016年06期

【摘要】：目的研究白樺Betula platyphylla 3-羥基-3-甲基戊二酰輔酶A還原酶(3-hydroxy-3-methyl-glutaryl-Co A reductase,Bp HMGR)全長基因結(jié)構(gòu)特征及編碼蛋白性質(zhì),揭示其節(jié)律表達(dá)模式以及對(duì)外源NO信號(hào)分子的響應(yīng)機(jī)制。方法通過RACE技術(shù)克隆白樺Bp HMGR基因,應(yīng)用生物信息學(xué)軟件對(duì)Bp HMGR及其編碼蛋白進(jìn)行詳細(xì)分析,利用實(shí)時(shí)熒光定量PCR定量分析Bp HMGR基因在晝夜節(jié)律變化中的表達(dá)模式。根據(jù)Bp HMGR氨基酸序列構(gòu)建系統(tǒng)進(jìn)化樹,分析晝夜節(jié)律以及外源NO對(duì)白樺葉片中Bp HMGR轉(zhuǎn)錄水平的影響。結(jié)果 Bp HMGR基因全長1 764 bp,包含完整開放閱讀框,已上傳至NCBI,并獲得登錄號(hào)KJ452334,其編碼蛋白由587 aa組成。白樺Bp HMGR基因與大豆(XP_003519474.1)、葡萄(XP_002275827.1)、苜蓿(XP_003617066.1)親緣關(guān)系較近。結(jié)論 Bp HMGR隨晝夜周期變化呈現(xiàn)出節(jié)律表達(dá)特性,在夜晚21:00時(shí)達(dá)到峰值。初步揭示了外源NO能誘導(dǎo)白樺細(xì)胞內(nèi)三萜代謝途徑的關(guān)鍵酶Bp HMGR基因上調(diào)表達(dá),進(jìn)而促進(jìn)三萜產(chǎn)物齊墩果酸的合成。
[Abstract]:Objective to study the structure characteristics and the properties of the full length gene of Betula platyphylla 3- hydroxyl -3- methyl amyl two acyl A reductase (3-hydroxy-3-methyl-glutaryl-Co A reductase, Bp HMGR), and to reveal the pattern of rhythmic expression and the response mechanism of foreign NO signal molecules. The Bp HMGR and its encoded protein were analyzed in detail with the bioinformatics software. The expression pattern of Bp HMGR gene in the circadian rhythm was quantified by real-time fluorescence quantitative PCR. The phylogenetic tree was constructed according to the sequence of Bp HMGR amino acids, and the influence of the circadian rhythm and the exogenous NO on the Bp HMGR transcriptional level in the leaves of Betula platyphylla were analyzed. The total length of Bp HMGR gene was 1764 BP, including a complete open reading frame, which had been uploaded to NCBI, and the login number KJ452334 was obtained. The encoding protein was composed of 587 AA. The genetic relationship between the Bp HMGR gene of Betula platyphylla and soybean (XP_003519474.1), grape (XP_002275827.1) and alfalfa (XP_003617066.1) was close. The characteristics, reaching the peak at 21:00 at night, preliminarily revealed that exogenous NO can induce the up-regulated expression of the key enzyme Bp HMGR gene of three terpene metabolic pathways in birch cells, thus promoting the synthesis of the three terpene product oleanolic acid.
【作者單位】： 東北林業(yè)大學(xué)生命科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(J1210053,31200463) 中央高�；究蒲袠I(yè)務(wù)費(fèi)專項(xiàng)資金資助(2572014DA04)
【分類號(hào)】：S792.153
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本文編號(hào)：1960130