本文選題：春秋兩季 + 不同地區(qū)�。� 參考：《中國農業(yè)科技導報》2017年05期

【摘要】：為了解新疆春秋兩季不同地區(qū)耐藥豬源大腸桿菌攜帶β-內酰胺酶和16S rRNA甲基化酶基因及其共存情況。通過PCR方法對克拉瑪依地區(qū)142株春季豬源耐藥菌、9株克拉瑪依秋季豬源耐藥菌、78株昌吉地區(qū)春季豬源耐藥菌和52株昌吉地區(qū)秋季豬源耐藥菌進行β-內酰胺酶(blaTEM、blaCMY-2、blaLAP-1、blaKPC、blaSHV和blaOXA)和16S rRNA甲基化酶(arm A和rmt B)基因檢測,并對檢出耐藥基因的PCR產物進行測序。結果顯示:克拉瑪依春季豬源耐藥菌攜帶的β-內酰胺酶基因為blaTEM(142/142,100%)、blaOXA(16/142,11.3%)、blaCMY-2(7/142,4.9%)和blaLAP-1(1/142,0.7%);克拉瑪依秋季豬源耐藥菌攜帶的β-內酰胺酶為blaTEM(9/9,100%)和blaOXA(1/9,11.1%),克拉瑪依春秋兩季豬源耐藥菌均未檢測出16S rRNA甲基化酶基因;昌吉地區(qū)春季豬源耐藥菌攜帶的β-內酰胺酶基因為blaTEM(78/78,100%)、blaOXA(9/78,11.5%)、blaCMY-2(3/78,3.8%)和16S rRNA甲基化酶基因rmt B(1/78,1.3%);昌吉地區(qū)秋季豬源耐藥菌攜帶的β-內酰胺酶基因為blaTEM(52/52,100%)、blaOXA(4/52,7.7%)、blaCMY-2(3/52,5.8%)和blaSHV(1/52,1.9%),未檢測出16S rRNA甲基化酶基因。上述結果表明春秋兩季不同地區(qū)豬源大腸桿菌blaTEM檢出率均為100%;其次不同地區(qū)春季豬源大腸桿菌對blaOXA檢出率均高于秋季豬源大腸桿菌。產生β-內酰胺酶及16S rRNA甲基化酶基因是豬源菌對β-內酰胺類及氨基糖苷類抗菌藥物耐藥的主要原因之一,養(yǎng)殖場應合理使用抗菌藥物,加強對產生β-內酰胺酶及16S rRNA甲基化酶基因的監(jiān)控。
[Abstract]:In order to understand the coexistence of 尾 -lactamase and 16s rRNA methylase gene in Escherichia coli from different areas of Xinjiang in spring and autumn. Application of 尾 -lactamase (尾 -lactamase) to 142 strains of spring porcine drug-resistant bacteria in Karamay region, 9 strains of klamayi autumn porcine drug-resistance bacteria, 78 strains of spring porcine drug-resistant bacteria in Changji area and 52 strains of autumn porcine drug-resistant bacteria in Changji area were used to detect 尾 -lactamase (尾 -lactamase) blaCMY-2 blaLAP-1blaKPCblaSHV and 尾 -lactamase (尾 -lactamase) blaCMY-2 blaLAP-1KPCblaSHV. Detection of blaOXA and 16s rRNA methylases ARMA and rmt B, The PCR products of drug resistance gene were sequenced. The results showed that the 尾 -lactamases carried by resistant strains from spring pigs in Karamay were as follows: blaTEM142 / 142100 because of blaTEM142P / 142100, 16 / 142OXAX / 16 / 1422.3X / 7 / 7 / 1424.9b) and blaLAP-1 / 1 / 1420.70.70.The 尾 -lactamases carried by resistant strains from pigs in Karamay fall were TEblaM9r9100m) and blaOXA1 / 911.1x, respectively, and the resistant bacteria from pig origin were not detected in Karamayi in spring and autumn. The 尾 -lactamases carried by drug-resistant bacteria from pig origin of Karamayi in autumn were TEblaM9 / 9100) and blaOXA1 / 9 / 9 / 11 / 1Q, respectively. 16s rRNA methylase gene was obtained. 鏄屽悏鍦板尯鏄ュ鐚簮鑰愯嵂鑿屾惡甯︾殑尾-鍐呴叞鑳洪叾鍩哄洜涓篵laTEM(78/78,100%),blaOXA(9/78,11.5%),blaCMY-2(3/78,3.8%)鍜，

本文編號：1947139