發(fā)布時(shí)間：2018-05-28 09:35

  本文選題：廣譜 + Pigm�。� 參考：《分子植物育種》2017年11期

【摘要】：為改良已選育的高產(chǎn)優(yōu)質(zhì)粳稻高代新品系的稻瘟病抗性,以引進(jìn)的攜帶有水稻廣譜抗稻瘟病基因Pigm的秈稻品種谷梅4號(hào)和粳稻材料MP3為供體,利用花粉管通道技術(shù)將其基因組DNA導(dǎo)入到SD-2、SD-3、SD-4、SD-5、SD-6、DJ13、ZD15、14PB1和14PB4等高代品系和品種寧粳43號(hào)中,利用Pigm基因的顯性標(biāo)記M26205和M80410對(duì)受體及獲得的導(dǎo)入系進(jìn)行檢測(cè),結(jié)果表明:在所有受體中,兩個(gè)標(biāo)記均未擴(kuò)增出條帶,說明所有受體本身都不含Pigm基因;在檢測(cè)的854株導(dǎo)入株系中,利用標(biāo)記M26205檢測(cè)總共有328株能夠擴(kuò)增出856 bp,或1 019 bp和856 bp的條帶,初步推斷這328個(gè)株系攜帶有Pigm基因,平均陽(yáng)性株率為40.51%;利用標(biāo)記M80410檢測(cè)總共有169株能夠擴(kuò)增出517 bp的條帶,推斷這169個(gè)株系可能攜帶有Pigm基因,平均陽(yáng)性株率為19.83%。選擇兩個(gè)標(biāo)記能同時(shí)檢測(cè)出的陽(yáng)性植株107株作為Pigm基因的導(dǎo)入系,進(jìn)行進(jìn)一步的加代培育和輔助選擇。
[Abstract]:In order to improve the blast resistance of the new high yield and good quality japonica rice lines, the introduced indica rice cultivar Gumei 4 carrying the broad-spectrum blast resistance gene Pigm and the japonica rice material MP3 were used as donors. The genomic DNA was introduced into high generation lines and cultivar Ningjing 43 by pollen tube pathway technique. The results showed that: among all the receptors, the dominant markers M26205 and M80410 of the Pigm gene were used to detect the receptors and the resulting lines. No bands were amplified from the two markers, indicating that none of the receptors contained Pigm gene, and a total of 328 of the 854 lines were detected by using marker M26205 to amplify 856 BP, or 1 019 BP and 856 BP bands. It was preliminarily inferred that these 328 strains carried Pigm gene, and the average positive rate was 40.51.The results showed that 169 strains could amplify 517bp bands by using marker M80410. It was inferred that the 169 lines might carry Pigm gene, and the average positive rate was 19.833%. 107 positive plants which could be detected simultaneously by two markers were selected as Pigm gene importing lines for further generation and selection.
【作者單位】： 寧夏農(nóng)林科學(xué)院農(nóng)業(yè)生物技術(shù)研究中心;寧夏大學(xué)農(nóng)學(xué)院;
【基金】：寧夏回族自治區(qū)農(nóng)業(yè)育種專項(xiàng)(2013NYYZ03) 寧夏農(nóng)林科學(xué)院科技創(chuàng)新先導(dǎo)資金項(xiàng)目(NKYJ-16-07;NKYQ-17-02)共同資助
【分類號(hào)】：Q943.2;S435.111.41

【相似文獻(xiàn)】

相關(guān)期刊論文 前3條

1 王昌明;水稻對(duì)稻瘟病抗性的遺傳[J];江西科學(xué);1986年03期

2 吳建利,莊杰云,李德葆,鄭康樂;水稻對(duì)稻瘟病抗性的分子生物學(xué)研究進(jìn)展[J];中國(guó)水稻科學(xué);1999年02期

3 周淑月;;利用水稻花藥培養(yǎng)篩選稻瘟病抗性突變體[J];溫州農(nóng)業(yè)科技;1985年02期

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本文編號(hào)：1946200