本文選題：豬種 + FUT基因��； 參考：《畜牧與獸醫(yī)》2017年01期

【摘要】：α(1,2)巖藻糖轉(zhuǎn)移酶基因1(α-1,2-Fucosyltransferase gene,FUT1)是可以調(diào)節(jié)大腸桿菌F18侵染豬小腸、并引起仔豬水腫和腹瀉的一個抗性基因。采用PCR-SSCP方法,檢測了FUT1基因在大白豬、長白豬、杜洛克、馬身豬、山西黑豬、晉汾白豬6個豬種中的多態(tài)性。結(jié)果表明:在大白豬、長白豬和杜洛克這3個引進(jìn)品種中,存在A、B、C 3種單倍型,A單倍型的平均分布頻率為0.58;而在地方品種馬身豬及其培育品種山西黑豬和晉汾白豬中只發(fā)現(xiàn)了單倍型A。經(jīng)測序比對發(fā)現(xiàn)B單倍型第5位堿基發(fā)生了C→A的轉(zhuǎn)換,第145個堿基處發(fā)生了T→C的突變;而單倍型C的第372位堿基A發(fā)生缺失。大白和杜洛克2個種群Hardy-Weinberg不平衡,FUT1基因在國外引進(jìn)豬種大白、長白、杜洛克種群多態(tài)性豐富。
[Abstract]:The 偽 -1n 2-Fucosyltransferase gene (FUT1) is a resistant gene that can regulate the infection of E. coli F18 into the small intestine of pigs and cause edema and diarrhea in piglets. The polymorphisms of FUT1 gene in 6 breeds of large white pig, Landrace pig, Duroc, Ma-Shen pig, Shanxi Black pig and Jinfen White pig were detected by PCR-SSCP method. The results showed that among the three introduced breeds, large white pig, Landrace pig and Duroc, The average distribution frequency of the three haplotypes was 0.58, but only haplotype A was found in the local breeds of Ma-Shen pig and its breeds Shanxi black pig and Jinfen white pig. After sequencing, it was found that the fifth base of B haplotype had a transition of C A, the mutation of T C at the 145 base, and the deletion of 372 base A of haplotype C. The Hardy-Weinberg disequilibrium FUT1 gene of two populations of large white and Duroc were rich in the populations of white, white and Duroc introduced from foreign countries.
【作者單位】： 山西農(nóng)業(yè)大學(xué)動物科技學(xué)院;山西省畜牧遺傳育種中心;山西省大同市種豬場;
【基金】：山西省高等學(xué)�？萍紕�(chuàng)新項目(2014129) 山西省科技攻關(guān)項目(041046,20140311020-5,20130311026-1) 山西省農(nóng)業(yè)科技成果轉(zhuǎn)化與推廣示范項目(SXNKTG09)
【分類號】：S828

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