發(fā)布時(shí)間：2018-05-25 18:53

  本文選題：香蕉枯萎病 + 花芽分化期�。� 參考：《生物技術(shù)通報(bào)》2017年07期

【摘要】：通過對(duì)‘巴西蕉’和‘寶島蕉’花芽分化期響應(yīng)枯萎病菌4號(hào)生理小種(Fusarium oxysporum f.sp.cubense race 4,Foc4)侵染的轉(zhuǎn)錄組進(jìn)行分析,獲得了大量差異表達(dá)基因。本研究以‘巴西蕉’和‘寶島蕉’花芽分化期根部組織為材料,采用實(shí)時(shí)熒光定量PCR(q PCR)方法測(cè)定了10個(gè)與特定抗病機(jī)制相關(guān)基因的表達(dá)變化。結(jié)果表明,10個(gè)基因在‘巴西蕉’和‘寶島蕉’花芽分化過程中均有表達(dá),但其表達(dá)量存在差異。與對(duì)照‘巴西蕉’相比,除laccase-4(A)基因在‘寶島蕉’中的表達(dá)量明顯下調(diào)外,laccase-4(B)、periplasmic beta-glucosidase precursor、glutathione-S-transferase Cla47、class Ⅲ peroxidase 136 precursor、peroxidase N、similar to T-phylloplanin、snakin-1、putative chitinase及cellulose synthase catalytic subunit 12等9個(gè)基因的表達(dá)量均上調(diào),其中peroxidase N基因的表達(dá)量呈極顯著上調(diào),相對(duì)表達(dá)量約為‘巴西蕉’的1 682倍。10個(gè)差異表達(dá)基因可能參與‘寶島蕉’花芽分化期對(duì)病原菌的防衛(wèi)反應(yīng),為進(jìn)一步探討‘寶島蕉‘復(fù)雜的抗病防御反應(yīng)分子機(jī)理奠定基礎(chǔ)。
[Abstract]:A large number of differentially expressed genes were obtained by analyzing the transcriptome of Fusarium oxysporum f.sp.cubense race 4 (Foc4), a physiological species of Fusarium oxysporum f.sp.cubense race 4, in response to the floral bud differentiation of 'Canna brasiliensis' and 'Baodao banana'. In this study, the expression changes of 10 genes related to specific disease resistance mechanism were determined by real-time fluorescence quantitative PCR(q method using the root tissue of 'Canna bassiana' and 'Baodao banana' during flower bud differentiation. The results showed that all of the 10 genes were expressed in the process of flower bud differentiation, but there were differences in their expression levels. Compared with the control, the expression of laccase-4) gene was significantly down-regulated in 'Baodao banana', except that the expression of laccase-4 beta-glucosidase precursorsortium-glutathione-S-transferase Cla47 class 鈪，

本文編號(hào)：1934288