本文選題：siRNA + RhoGDI　； 參考：《中國免疫學雜志》2017年04期

【摘要】：目的:應用小分子干擾RNA(siRNA)沉默RhoGDI2基因的表達,初步探討其對結(jié)腸癌細胞增殖、遷移能力的影響及可能的機制。方法:分別運用Western blot和RT-q PCR檢測結(jié)腸癌細胞株RKO、HT29、SW620、SW480、HCT116基因RhoGDI2的表達情況。設(shè)計并合成RhoGDI2 siRNA干擾序列,按照LipofectamineTM2000轉(zhuǎn)染方法將siRNA干擾序列轉(zhuǎn)染到目的細胞,設(shè)置實驗干擾組、空白對照和陰性對照組;CCK-8實驗檢測細胞增殖能力,細胞劃痕試驗和Transwell實驗檢測細胞遷移、侵襲能力。結(jié)果:人結(jié)腸癌細胞株RhoGDI2表達量由高到低依次是RKO、HT29、SW620、SW480、HCT116;RKO細胞siRNA干擾后RhoGDI2表達抑制率大于70%:實驗干擾組、陰性對照組、空白對照組細胞增殖率分別是(0.683±0.013)、(0.866±0.088)、(0.905±0.008),P0.05;實驗干擾組細胞遷移、侵襲速率較對照組均減慢。沉默RhoGDI2基因的表達,實驗組細胞E-Cadherin較對照組表達量高,Vimentin蛋白表達下降。結(jié)論:結(jié)腸癌RhoGDI2基因沉默可能通過抑制EMT進程阻止腫瘤惡性生物學行為。
[Abstract]:Aim: to silence the expression of RhoGDI2 gene by small interfering RNAs (siRNAs), and to explore the possible mechanism of its effect on the proliferation and migration of colon cancer cells. Methods: Western blot and RT-q PCR were used to detect the RhoGDI2 expression of HCT116 gene. The RhoGDI2 siRNA interference sequence was designed and synthesized, and the siRNA interference sequence was transfected into the target cell according to the LipofectamineTM2000 transfection method. The experimental interference group, the blank control group and the negative control group were used to detect the proliferation ability of the cells. Cell scratch test and Transwell assay were used to detect cell migration and invasion. Results: the order of RhoGDI2 expression in human colon cancer cell line was RKOHT29, SW620, SW480, HCT116, RKO cells, the inhibition rate of RhoGDI2 expression was higher than 70: the proliferative rate of experimental interference group, negative control group and blank control group was 0. 683 鹵0. 083 鹵0. 0888 鹵0. 905 鹵0. 008P0.05. the cell migration of experimental interference group, negative control group and blank control group were 0. 683 鹵0. 088 鹵0. 905 鹵0. 008P0. 05 respectively. The invasion rate was slower than that of the control group. Silencing the expression of RhoGDI2 gene, the expression of Vimentin protein in the experimental group was significantly lower than that in the control group. Conclusion: RhoGDI2 gene silencing in colon cancer may prevent malignant biological behavior by inhibiting EMT progression.
【作者單位】： 吉林大學第二醫(yī)院肝膽胰內(nèi)科;第二軍醫(yī)大學長海醫(yī)院檢驗科;上海交通大學醫(yī)學院附屬同仁醫(yī)院腫瘤科;
【基金】：吉林省科技廳科研基金課題(2013c026-1)
【分類號】：R735.35

【相似文獻】

相關(guān)期刊論文 前10條

1 王毅;王芳;趙宏宇;王陽;周余來;原田守;山田亮;伊東恭悟;;前列腺酸性磷酸酶——結(jié)腸癌主動免疫治療新靶點[J];中國老年學雜志;2008年18期

2 邵世和,謝立蘋,霍龍;硒與結(jié)腸癌研究進展[J];北華大學學報(自然科學版);2003年05期

3 程文芳,顧曉紅,錢偉,易粹瓊;結(jié)腸癌中腫瘤壞死因子相關(guān)凋亡誘導配體受體的表達[J];胃腸病學;2003年02期

4 郭艷麗,閆慧明,宋善俊;組織因子在結(jié)腸癌表達的臨床意義(英文)[J];中國現(xiàn)代醫(yī)學雜志;2005年15期

5 李建國;王雙燕;沈若武;王守彪;;血管內(nèi)皮生長因子與結(jié)腸癌的相關(guān)性研究[J];山東醫(yī)藥;2006年07期

6 孫軍;沈磊;羅和生;沈志祥;;結(jié)腸癌瘦素和瘦素受體表達及其意義[J];中華消化雜志;2006年08期

7 宋e，

本文編號：1927418