本文選題：鈣 + 鉛�。� 參考：《營(yíng)養(yǎng)學(xué)報(bào)》2017年01期

【摘要】：目的通過(guò)研究鈣對(duì)染鉛大鼠海馬NMDAR和m Glu R5受體介導(dǎo)相關(guān)基因mRNA及CaMKII蛋白表達(dá)的影響,為進(jìn)一步以補(bǔ)鈣預(yù)防鉛的危害提供理論依據(jù)。方法選擇體質(zhì)量(50±2)g的清潔級(jí)雄性SD大鼠60只,共分五組,每組12只,分別為對(duì)照組(基礎(chǔ)飼料,蒸餾水)、染鉛組(基礎(chǔ)飼料,0.2%醋酸鉛飲水)、試驗(yàn)Ⅰ組(基礎(chǔ)飼料添加0.5%碳酸鈣,0.2%醋酸鉛飲水)、試驗(yàn)Ⅱ組(基礎(chǔ)飼料添加1%碳酸鈣,0.2%醋酸鉛飲水)和試驗(yàn)Ⅲ組(基礎(chǔ)飼料添加2%碳酸鈣,0.2%醋酸鉛飲水)。連續(xù)飼養(yǎng)60 d后,取海馬,用熒光定量RT-PCR法測(cè)定海馬中Grm5、Camk2a、Grin1、Homer1、Prkca和ERK2的mRNA表達(dá)量,Western-blotting測(cè)定大鼠海馬中CaMKII的蛋白表達(dá)量。結(jié)果與對(duì)照組相比,染鉛組海馬中Grm5、Camk2a、Grin1、Homer1、Prkca、ERK2的mRNA表達(dá)量及CaMKII的蛋白表達(dá)量均極顯著降低(P0.01)。與染鉛組相比,試驗(yàn)組鈣濃度在一定范圍內(nèi)越高,目的基因表達(dá)量相對(duì)升高越多,試驗(yàn)Ⅱ組CaMKII的蛋白表達(dá)量極顯著上升(P0.01)。結(jié)論一定濃度的鈣可干預(yù)鉛致大鼠海馬組織的損傷。
[Abstract]:Objective to study the effect of calcium on the expression of NMDAR and m Glu R5 receptor mediated genes mRNA and CaMKII in hippocampus of lead exposed rats, and to provide theoretical basis for calcium supplementation to prevent the harm of lead. Methods Sixty male SD rats of clean grade with 50 鹵2g body weight were divided into five groups, 12 rats in each group, as control group. Distilled water, lead group (basic feed 0.2% lead acetate drinking water), test I group (basic feed supplemented with 0.5% calcium carbonate 0.2wt% lead acetate drinking water), test 鈪，

本文編號(hào)：1924725