發(fā)布時(shí)間：2018-05-23 06:35

  本文選題：降鈣素基因相關(guān)肽 + 杏仁核中央核區(qū)��； 參考：《生理學(xué)報(bào)》2017年02期

【摘要】：本研究旨在探討外源性給予杏仁核中央核區(qū)不同濃度的降鈣素基因相關(guān)肽(calcitonin gene-related peptide,CGRP)對(duì)小鼠認(rèn)知和學(xué)習(xí)記憶的影響。將30日齡C57BL/6J小鼠隨機(jī)分為空白對(duì)照組、手術(shù)對(duì)照組、200 ng CGRP組、400 ng CGRP組和800 ng CGRP組,每組10只。外源性給予雙側(cè)杏仁核中央核區(qū)不同濃度的CGRP后,用曠場(chǎng)實(shí)驗(yàn)檢測(cè)各組小鼠認(rèn)知功能,用新物體認(rèn)知實(shí)驗(yàn)和Morris水迷宮實(shí)驗(yàn)檢測(cè)各組小鼠學(xué)習(xí)記憶能力。曠場(chǎng)實(shí)驗(yàn)結(jié)果顯示,與空白對(duì)照組相比,800 ng CGRP組小鼠的水平運(yùn)動(dòng)得分顯著增加。新物體認(rèn)知實(shí)驗(yàn)結(jié)果顯示,與空白對(duì)照組相比,400 ng CGRP組的認(rèn)知指數(shù)顯著提高。Morris水迷宮實(shí)驗(yàn)結(jié)果顯示,與空白對(duì)照組比較,400和800 ng CGRP組小鼠的潛伏期均顯著縮短,穿越平臺(tái)的次數(shù)顯著增加;與200 ng CGRP組比較,400和800 ng CGRP組小鼠尋找平臺(tái)的潛伏期均明顯縮短。以上結(jié)果提示,雙側(cè)杏仁核注射CGRP可劑量依賴性促進(jìn)小鼠非空間和空間學(xué)習(xí)記憶功能。
[Abstract]:The purpose of this study was to investigate the effects of exogenous administration of calcitonin gene-related peptidea on cognition, learning and memory of mice in the central amygdaloid nucleus region with different concentrations of calcitonin gene related peptide (calcitonin gene-related). The 30-day-old C57BL/6J mice were randomly divided into control group (n = 10), operation control group (n = 10), control group (n = 10) and control group (n = 10). After exogenous administration of different concentrations of CGRP in the central nucleus of amygdala, the cognitive function of each group was tested by open field test, and the learning and memory ability of each group was measured by new object cognition experiment and Morris water maze test. The results of open field test showed that the horizontal exercise scores of mice in the 800ng CGRP group were significantly higher than those in the blank control group. The results of new object cognition test showed that compared with the control group, the cognitive index of the 400ng CGRP group was significantly higher than that of the control group. The results showed that compared with the blank control group, the latency of mice in the 400ng CGRP group was significantly shorter than that in the control group. Compared with 200ng CGRP group, the latency of searching for platform was significantly shortened in mice of 400ng CGRP group and 800ng CGRP group. These results suggest that bilateral amygdala injection of CGRP can promote spatial and spatial learning and memory function in a dose-dependent manner.
【作者單位】： 河南中醫(yī)藥大學(xué)基礎(chǔ)醫(yī)學(xué)院生理學(xué)學(xué)科;
【基金】：supported by the National Natural Science Foundation of China(No.81373852) the Key Scientific Research Project of Colleges and Universities in Henan Province,China(No.15A310019) PhD Research Fund of Henan University of Traditional Chinese Medicine,China(No.BSJJ-201510)
【分類號(hào)】：R338

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 唐開福,張銀輝,袁建剛;CaMKⅡ在學(xué)習(xí)和記憶中的作用[J];生物工程進(jìn)展;2001年02期

2 沈楠,金麗梅,顧饒勝,王艷春,吳曉東,李平;雌二醇對(duì)小鼠學(xué)習(xí)記憶的影響[J];第四軍醫(yī)大學(xué)吉林軍醫(yī)學(xué)院學(xué)報(bào);2000年02期

3 霍展樣,樊愛英,劉曉麗,邱學(xué)才;褪黑素對(duì)嗎啡戒斷小鼠學(xué)習(xí)記憶及海馬CA1區(qū)細(xì)胞數(shù)的影響[J];新鄉(xiāng)醫(yī)學(xué)院學(xué)報(bào);2004年04期

4 聶榮慶,張進(jìn),胡國柱,吳小鑒,文珠,吳東風(fēng);神經(jīng)活性物質(zhì)對(duì)小鼠學(xué)習(xí)記憶能力及耐力的影響[J];中國康復(fù)理論與實(shí)踐;2003年01期

5 周國興,王春旭,劉正清;熱應(yīng)激預(yù)適應(yīng)對(duì)小鼠學(xué)習(xí)記憶的影響[J];解剖學(xué)研究;2004年04期

6 陳蓉,肖信生,王新光;Org-2766對(duì)小鼠學(xué)習(xí)記憶的影響及其與腦內(nèi)蛋白質(zhì)合成的關(guān)系[J];心理學(xué)報(bào);1990年03期

7 章子貴,杜紅燕,姜建明,吳馥梅;衰老過程中小鼠學(xué)習(xí)記憶與腦內(nèi)突觸結(jié)構(gòu)參數(shù)變化的相關(guān)性[J];神經(jīng)解剖學(xué)雜志;1998年02期

8 章子貴;陳燕珍;申秀英;許曉路;黃香芝;;稀土元素釤對(duì)小鼠學(xué)習(xí)記憶的影響及其腦內(nèi)機(jī)制[J];浙江師范大學(xué)學(xué)報(bào)(自然科學(xué)版);2007年03期

9 邵鄰相,徐麗珊,丁淑靜;饑餓脅迫對(duì)小鼠學(xué)習(xí)記憶、SOD和心肌丙二醛的影響[J];動(dòng)物學(xué)雜志;2002年06期

10 陸漢新，王革平，戴君勇，吳馥梅;Mg~(2+)對(duì)小鼠學(xué)習(xí)記憶的影響及其與海馬CA_3區(qū)和大腦皮層突觸界面結(jié)構(gòu)的相關(guān)性[J];神經(jīng)科學(xué);1996年03期

相關(guān)碩士學(xué)位論文 前5條

1 孫靜麗;文冠果皂甙對(duì)小鼠學(xué)習(xí)記憶功能及海馬內(nèi)膽堿酯酶和頜下腺內(nèi)神經(jīng)生長因子活性的影響[D];中國醫(yī)科大學(xué);2002年

2 丁琳琳;殼寡糖螯合鋅對(duì)小鼠學(xué)習(xí)記憶能力影響的比較研究[D];遼寧師范大學(xué);2013年

3 唐莉莉;豐富和貧瘠環(huán)境對(duì)老齡雌性小鼠學(xué)習(xí)記憶的影響[D];華中師范大學(xué);2009年

4 呂雙瑜;C75對(duì)小鼠學(xué)習(xí)記憶的影響及apelin-13對(duì)胃排空和小腸通過的作用[D];蘭州大學(xué);2011年

5 陳燕珍;亞慢性釤染毒對(duì)小鼠學(xué)習(xí)記憶的影響及其腦內(nèi)機(jī)制的研究[D];浙江師范大學(xué);2006年

，

本文編號(hào)：1923650