本文選題：檸條錦雞兒 + CkFH基因��； 參考：《廣西植物》2017年06期

【摘要】：檸條錦雞兒為豆科灌木,對各種環(huán)境脅迫具有較強(qiáng)的適應(yīng)能力,類黃酮是天然的抗氧化劑,花青素屬類黃酮化合物,逆境脅迫會影響植物體內(nèi)花青素的合成,而黃烷酮3-羥化酶(F3H)是花青素生物合成所必需的一種關(guān)鍵酶。該研究成功分離克隆了檸條錦雞兒的F3H基因,命名為CkF3H。CkF3H基因的開放閱讀框(ORF)為1095 bp,編碼364個氨基酸,推測的蛋白質(zhì)分子量為41.3 kDa,理論等電點為5.9。生物信息學(xué)分析發(fā)現(xiàn),CkF3H基因序列與其它植物F3H有較高的一致性,推測CkF3H蛋白與其它植物F3H蛋白具有相似的功能。利用染色體步移法克隆得到CkF3H起始密碼子ATG上游468 bp的啟動子序列,PlantCARE軟件分析表明,該序列具有啟動子的基本元件CAAT-box和TATA-box以及多種與逆境脅迫相關(guān)的順式調(diào)控元件。實時熒光定量PCR分析表明,CkF3H在檸條的根、莖和葉中均有表達(dá),沒有組織特異性;CkF3H的表達(dá)受低溫、高鹽、干旱和高溫脅迫的誘導(dǎo),并且在低溫脅迫下,CkF3H的表達(dá)還受到光周期的影響。綜上所述,研究結(jié)果表明CkF3H基因在檸條錦雞兒適應(yīng)低溫、高鹽、干旱和高溫脅迫的過程中發(fā)揮作用。
[Abstract]:Caragana korshinskii is a legume shrub. Flavonoids are natural antioxidants and anthocyanins are flavonoids. Stress affects the synthesis of anthocyanins in plants. F3H) is a key enzyme for anthocyanin biosynthesis. In this study, the F3H gene of Caragana korshinskii was successfully isolated and cloned. The open reading frame named CkF3H.CkF3H gene was 1095 BP, encoding 364 amino acids. The deduced molecular weight of protein was 41.3 kDa and the theoretical isoelectric point was 5.9. Bioinformatics analysis showed that the sequence of CkF3H gene was consistent with that of other plants. It was suggested that CkF3H protein had similar function with other plant F3H proteins. A 468bp promoter sequence upstream of CkF3H initiation codon (ATG) was cloned by chromosome step method and analyzed by PlantCARE software. The results showed that the promoter had the basic components of promoter CAAT-box and TATA-box, as well as a variety of cis-regulatory elements related to stress. Real-time fluorescence quantitative PCR analysis showed that CkF3H was expressed in roots, stems and leaves of Caragana korshinskii, and no tissue specific CkF3H expression was induced by low temperature, high salt, drought and high temperature stress. The expression of CkF3H was also affected by photoperiod under low temperature stress. In conclusion, CkF3H gene plays an important role in the adaptation of Caragana korshinskii to low temperature, high salt, drought and high temperature stress.
【作者單位】： 西北師范大學(xué)生命科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金(31460099) 西北師范大學(xué)青年教師科研能力提升計劃項目(NWNU-LKQN-14-23)~~
【分類號】：Q943.2

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