本文選題：光唇魚(yú) + 肌肉生長(zhǎng)抑制素基因(MSTN)�。� 參考：《農(nóng)業(yè)生物技術(shù)學(xué)報(bào)》2017年05期

【摘要】：肌肉生長(zhǎng)抑制素(myostatin,MSTN)是骨骼肌生長(zhǎng)的負(fù)調(diào)控因子,MSTN基因在遺傳育種上有很大的潛在應(yīng)用價(jià)值。光唇魚(yú)(Acrossocheilus fasciatus)是一種具有較大開(kāi)發(fā)前景的經(jīng)濟(jì)魚(yú)類,但其生長(zhǎng)速度較慢。為了解光唇魚(yú)MSTN基因的特征并為生長(zhǎng)性狀標(biāo)記輔助選擇提供基礎(chǔ)資料,本研究克隆了光唇魚(yú)MSTN c DNA和基因組DNA序列、檢測(cè)了組織表達(dá)情況并進(jìn)行了單核苷酸多態(tài)性(single nucleotide polymorphism,SNP)位點(diǎn)篩查。結(jié)果表明,光唇魚(yú)MSTN c DNA序列全長(zhǎng)2 163 bp,含有一個(gè)1 128 bp的開(kāi)放閱讀框(open reading frame,ORF)、編碼375個(gè)氨基酸,預(yù)測(cè)的編碼蛋白具有脊椎動(dòng)物MSTN的序列特征(如含有1個(gè)蛋白酶水解位點(diǎn)RIRR、C端有9個(gè)保守的半胱氨酸殘基)。基于MSTN氨基酸序列的比對(duì)顯示不同魚(yú)類之間MSTN序列同源性較高(80%),而魚(yú)類與其他脊椎動(dòng)物同源性較低(65%左右);光唇魚(yú)與其他鯉科魚(yú)類的MSTN基因高度同源(96.5%)。系統(tǒng)進(jìn)化樹(shù)中魚(yú)類聚為一枝、其他脊椎動(dòng)物聚為另一枝,光唇魚(yú)MSTN位于鯉科魚(yú)類的小簇中。半定量RT-PCR檢測(cè)顯示,MSTN m RNA在光唇魚(yú)腦和肌肉中高表達(dá)、腸和肝臟中有微弱表達(dá)、其他組織中均未見(jiàn)表達(dá)。光唇魚(yú)MSTN基因的DNA序列全長(zhǎng)5 828bp,含有3個(gè)外顯子和2個(gè)內(nèi)含子,在第二內(nèi)含子中檢測(cè)到10個(gè)SNP位點(diǎn),但與生長(zhǎng)性狀(體重、體長(zhǎng))不存在顯著相關(guān)性(P0.05)。研究結(jié)果為探究光唇魚(yú)MSTN基因的功能和篩選生長(zhǎng)性狀相關(guān)輔助育種標(biāo)記提供了參考資料。
[Abstract]:The MSTN gene, a negative regulatory factor for skeletal muscle growth, has great potential application value in genetics and breeding. Acrossocheilus fasciatus is a kind of economic fish with great development prospect, but its growth rate is slow. In order to understand the characteristics of MSTN gene and provide basic data for marker-assisted selection of growth traits, MSTN c DNA and genomic DNA sequences were cloned. Tissue expression was detected and single nucleotide polymorphisms (SNPs) loci were screened. The results showed that the MSTN c DNA sequence was 2 163 BP in length and contained a 1 128 BP open reading frame ORF, encoding 375 amino acids. The predicted protein has the sequence characteristics of vertebrate MSTN (such as 9 conserved cysteine residues at the C terminal of RIRRN containing a protease hydrolysis site). Alignment based on MSTN amino acid sequence showed that the homology of MSTN sequence was high among different fishes, but the homology between fish and other vertebrates was about 65%, and the MSTN gene homology of Lip fish and other Cyprinidae fishes was 96.5%. The fish in phylogenetic tree are clustered into one branch and the other vertebrates in the other branch. The MSTN is located in the small cluster of Cyprinidae. The results of semi-quantitative RT-PCR showed that the expression of MSTN m RNA was high in brain and muscle, weak in intestine and liver, and not in other tissues. The DNA sequence of MSTN gene was 5 828 BP, containing 3 exons and 2 introns. Ten SNP loci were detected in the second intron, but there was no significant correlation with growth traits (body weight, body length). The results provided a reference for exploring the function of MSTN gene and screening related auxiliary breeding markers for growth traits.
【作者單位】： 寧波大學(xué)教育部應(yīng)用海洋生物技術(shù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家星火計(jì)劃項(xiàng)目(No.2011GA701001) 浙江省海洋與漁業(yè)項(xiàng)目(浙海漁計(jì)[2012]83號(hào)) 浙江省科技廳重大科技專項(xiàng)遺傳育種項(xiàng)目(No.2012C12907)
【分類號(hào)】：Q78;S917.4

【相似文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 閆錦錦;光唇魚(yú)和寬鰭渞線粒體全序列測(cè)定及分析[D];安徽師范大學(xué);2014年

，

本文編號(hào)：1897865