本文選題：青天葵 + 轉(zhuǎn)錄組　； 參考：《中藥新藥與臨床藥理》2017年05期

【摘要】：目的在青天葵球莖和葉片兩個組織的轉(zhuǎn)錄組測序數(shù)據(jù)的基礎上,進行差異表達基因(differential expressed gene,DEG)的篩選和分析。方法采用Reads Per Kb per reads(RPKM)方法計算青天葵轉(zhuǎn)錄組測序獲得的單基因(unigene)在球莖和葉片中表達量,根據(jù)球莖和葉片之間RPKM的log2倍數(shù)絕對值大于1且錯誤發(fā)現(xiàn)率小于0.01篩選DEG,將DEG歸類至Nr、GO、KEGG等公共數(shù)據(jù)庫獲取其功能釋義;并基于功能注釋,挖掘參與青天葵激素合成和信號傳導過程的DEG。結(jié)果篩選出符合條件的DEG共62 605條,包括了31 488條表達上調(diào)DEG和31 117條表達下調(diào)DEG。分別有51 652條和14 215條DEG在GO和KEGG數(shù)據(jù)庫中獲得注釋,涵蓋GO數(shù)據(jù)庫的42個功能亞類和KEGG數(shù)據(jù)庫的280條代謝途徑。在挖掘到的16個與青天葵激素合成和信號傳導過程的DEG中,7個為上調(diào)表達基因,9個為下調(diào)表達基因。結(jié)論通過轉(zhuǎn)錄組測序數(shù)據(jù)較全面地提供了青天葵球莖和葉片基因的差異表達情況,為青天葵的功能基因的克隆和功能分析等提供研究基礎和理論依據(jù)。
[Abstract]:Objective to screen and analyze the differentially expressed gene on the basis of the transcriptome sequencing data of the corms and leaves of Agaricum davidii. Methods Reads Per Kb per RPKM method was used to calculate the expression of single gene Onunigenein in bulbs and leaves. According to the absolute value of log2 multiple of RPKM between bulb and leaf is greater than 1 and the error detection rate is less than 0. 01, the DEG is classified into public databases such as NrrngogokEGG to obtain its function interpretation, and based on the function annotation, Dig out the DEG involved in hormone synthesis and signal transduction. Results 62,605 DEG were screened, including 31,488 up-regulated DEG and 31,117 down-regulated DEG. 51,652 and 14,215 DEG were annotated in go and KEGG databases, covering 42 functional subclasses in go database and 280 metabolic pathways in KEGG database. Of the 16 extracted DEG associated with hormone synthesis and signal transduction, 7 were up-regulated and 9 down-regulated. Conclusion the differentially expressed genes in the corms and leaves of Agaricus davidii were fully provided by the transcriptional sequencing data, which provided the basis and theoretical basis for cloning and functional analysis of functional genes.
【作者單位】： 廣東醫(yī)科大學;廣州中醫(yī)藥大學中藥資源科學與工程研究中心嶺南中藥資源教育部重點實驗室國家中成藥工程技術研究中心南藥研發(fā)實驗室;
【基金】：廣東省自然科學基金博士啟動項目(2015A030310519)
【分類號】：Q943.2;S567.239

【相似文獻】

相關期刊論文 前2條

1 潘學峰,符式欽,戴衛(wèi)端;青天葵葉片離體培養(yǎng)及植株再生[J];海南大學學報(自然科學版);2001年04期

2 黃紅兵,朱品業(yè),何麗清,鄧海鳴;青天葵的薄層色譜鑒別與總氨基酸含量測定[J];中國野生植物資源;1999年04期

，

本文編號：1895154