本文選題：海島棉 + GbWRKY　； 參考：《棉花學(xué)報(bào)》2017年04期

【摘要】：【目的】WRKY轉(zhuǎn)錄因子調(diào)控多種生物學(xué)進(jìn)程,包括植物生長(zhǎng)發(fā)育和應(yīng)答多種環(huán)境脅迫。本研究旨在分析WRKY轉(zhuǎn)錄因子在海島棉纖維發(fā)育中的功能�！痉椒ā繌暮u棉中克隆了1個(gè)WRKY轉(zhuǎn)錄因子基因GbWRKY40,進(jìn)行同源性分析、多序列比對(duì),利用熒光定量聚合酶鏈?zhǔn)椒磻?yīng)分析其表達(dá)模式,通過(guò)構(gòu)建酵母表達(dá)載體并轉(zhuǎn)化酵母菌株AH109研究其轉(zhuǎn)錄激活活性�！窘Y(jié)果】該基因c DNA全長(zhǎng)1713 bp,5'非編碼區(qū)長(zhǎng)261 bp,3'非編碼區(qū)長(zhǎng)510 bp;開(kāi)放閱讀框長(zhǎng)942 bp,編碼313個(gè)氨基酸,預(yù)測(cè)相對(duì)分子質(zhì)量約為34.138×103,等電點(diǎn)為8.46,包含5個(gè)外顯子和4個(gè)內(nèi)含子;其編碼蛋白含有1個(gè)WRKY保守區(qū)(WRKYGQK)和1個(gè)鋅指基序(C-X5-C-X23-H-X1-H),屬于WRKY家族第Ⅱ類(lèi)a組,包含3個(gè)核定位信號(hào)區(qū),與陸地棉GhWRKY40同源性最高。GbWRKY40在根和開(kāi)花后25 d纖維中表達(dá)量高,而且不具有轉(zhuǎn)錄激活活性�！窘Y(jié)論】GbWRKY40可能參與調(diào)控棉纖維次生壁發(fā)育。
[Abstract]:Objective: WRKY transcription factors regulate many biological processes, including plant growth and response to multiple environmental stresses. The purpose of this study was to analyze the function of WRKY transcription factor in the fiber development of island cotton. [methods] A WRKY transcription factor gene GbWRKY40 was cloned from island cotton for homology analysis and multiple sequence alignment. Fluorescence quantitative polymerase chain reaction (FQPCR) was used to analyze its expression pattern. By constructing yeast expression vector and transforming yeast strain AH109, the transcriptional activation activity was studied. [results] the total length of c DNA was 1713 BP / 5'non-coding region 1713 BP / 3', and the length of open reading frame was 942 BP, encoding 313 amino acids. The predicted molecular weight is about 34.138 脳 103, the isoelectric point is 8.46, including 5 exons and 4 introns, and the encoded protein contains one WRKY conserved region WRKYGQK) and one zinc finger motif, C-X5-C-X23-H-X1-HN, which belongs to the second class a group of the WRKY family and contains three nuclear localization signal regions. GbWRKY40 had the highest homology with upland cotton GhWRKY40 and had high expression in root and 25 days after anthesis, and had no transcriptional activation activity. [conclusion] GbWRKY40 may be involved in the regulation of cotton fiber secondary wall development.
【作者單位】： 新疆農(nóng)業(yè)大學(xué)農(nóng)學(xué)院/新疆農(nóng)業(yè)大學(xué)農(nóng)業(yè)生物技術(shù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：基金項(xiàng)目:新疆維吾爾自治區(qū)青年科技創(chuàng)新人才培養(yǎng)工程優(yōu)秀青年科技人才(qn2015yx012)
【分類(lèi)號(hào)】：S562
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本文編號(hào)：1894298