本文選題：轉(zhuǎn)基因大豆品系 + 環(huán)介導(dǎo)等溫擴增(LAMP)�。� 參考：《農(nóng)業(yè)生物技術(shù)學(xué)報》2017年02期

【摘要】：隨著全球商品化生產(chǎn)的轉(zhuǎn)基因產(chǎn)品日益增多,轉(zhuǎn)基因品系檢測已逐漸成為國內(nèi)及國際上轉(zhuǎn)基因成分檢測的發(fā)展趨勢。為實施我國轉(zhuǎn)基因農(nóng)產(chǎn)品的進出境、環(huán)境釋放及標簽標識等相關(guān)法規(guī),實現(xiàn)進口轉(zhuǎn)基因大豆(Glycine max)的有效管理,本研究針對DAS-81419-2、DP356043-5、A2704-12、FG72、BPSCV127-9和MON89788等6種常見轉(zhuǎn)基因大豆的旁側(cè)序列,分別設(shè)計了環(huán)介導(dǎo)等溫擴增(loop-mediated isothermal amplification,LAMP)引物。通過引物篩選及方法的特異性、靈敏度及檢出限驗證,建立了轉(zhuǎn)基因大豆DAS-81419-2、DP-356043、A2704-12、FG72、BPS-CV127-9和MON89788的LAMP檢測方法。結(jié)果表明,所建立的方法能特異、穩(wěn)定地檢測轉(zhuǎn)基因大豆DAS-81419-2、DP356043-5、A2704-12、FG72、BPSCV127-9和MON89788,檢測靈敏度可達到0.1%(W/W),通過在反應(yīng)終產(chǎn)物中加入SYBR GreenⅠ染料,用肉眼即可直接判斷檢測結(jié)果,檢測時間在30 min以內(nèi)。本方法快速準確,且不需要復(fù)雜昂貴的儀器設(shè)備,適用于檢測機構(gòu)及相關(guān)執(zhí)法部門對轉(zhuǎn)基因產(chǎn)品的快速檢測。
[Abstract]:With the increasing number of GM products commercialized in the world, the detection of GMOs has gradually become the trend of the detection of GMOs both at home and abroad. In order to implement the relevant laws and regulations on entry and exit, environmental release and labelling of genetically modified agricultural products in China, and to realize the effective management of imported transgenic soybean Glycine max, this study focused on the flanking sequences of six common transgenic soybeans, such as DAS-81419-2 DP356043-5, A2704-12, FG72BPSCV127-9 and MON89788, etc. Loop-mediated isothermal amplification primers were designed respectively. The detection methods of DAS-81419-2DP-356043A2704-12 FG72BPS-CV127-9 and MON89788 for transgenic soybean DAS-81419-2DAS-81419-2DP-356043A2704-127-9 and MON89788 were established by screening primers, specificity, sensitivity and detection limit. The results showed that the method was specific and stable for the detection of transgenic soybean DAS-81419-2DAS-81419-2dp356043-5 A2704-12FG72BPSCV127-9 and MON89788. The sensitivity of the method could reach 0.1% W / WN. By adding SYBR Green 鈪，

本文編號：1890330